Human TARDBP Gene cDNA Clone / ORF Clone

TARDBP cDNA Clone Datasheet

TARDBP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007375.3
• cDNA Size: 1245
• Gene Synonym: ALS10, TDP-43, TARDBP
• Source: Human

TARDBP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens TAR DNA binding protein DNA.
• Vector: pGEM-T Vector
  
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 423 T/C not causing the amino acid variation.

TARDBP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TARDBP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TARDBP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTGAAT ATATTCGGGT AACCGAAGAT GAGAACGATG AGCCCATTGA AATACCATCG
61       GAAGACGATG GGACGGTGCT GCTCTCCACG GTTACAGCCC AGTTTCCAGG GGCGTGTGGG
121      CTTCGCTACA GGAATCCAGT GTCTCAGTGT ATGAGAGGTG TCCGGCTGGT AGAAGGAATT
181      CTGCATGCCC CAGATGCTGG CTGGGGAAAT CTGGTGTATG TTGTCAACTA TCCAAAAGAT
241      AACAAAAGAA AAATGGATGA GACAGATGCT TCATCAGCAG TGAAAGTGAA AAGAGCAGTC
301      CAGAAAACAT CCGATTTAAT AGTGTTGGGT CTCCCATGGA AAACAACCGA ACAGGACCTG
361      AAAGAGTATT TTAGTACCTT TGGAGAAGTT CTTATGGTGC AGGTCAAGAA AGATCTTAAG
421      ACCGGTCATT CAAAGGGGTT TGGCTTTGTT CGTTTTACGG AATATGAAAC ACAAGTGAAA
481      GTAATGTCAC AGCGACATAT GATAGATGGA CGATGGTGTG ACTGCAAACT TCCTAATTCT
541      AAGCAAAGCC AAGATGAGCC TTTGAGAAGC AGAAAAGTGT TTGTGGGGCG CTGTACAGAG
601      GACATGACTG AGGATGAGCT GCGGGAGTTC TTCTCTCAGT ACGGGGATGT GATGGATGTC
661      TTCATCCCCA AGCCATTCAG GGCCTTTGCC TTTGTTACAT TTGCAGATGA TCAGATTGCG
721      CAGTCTCTTT GTGGAGAGGA CTTGATCATT AAAGGAATCA GCGTTCATAT ATCCAATGCC
781      GAACCTAAGC ACAATAGCAA TAGACAGTTA GAAAGAAGTG GAAGATTTGG TGGTAATCCA
841      GGTGGCTTTG GGAATCAGGG TGGATTTGGT AATAGCAGAG GGGGTGGAGC TGGTTTGGGA
901      AACAATCAAG GTAGTAATAT GGGTGGTGGG ATGAACTTTG GTGCGTTCAG CATTAATCCA
961      GCCATGATGG CTGCCGCCCA GGCAGCACTA CAGAGCAGTT GGGGTATGAT GGGCATGTTA
1021     GCCAGCCAGC AGAACCAGTC AGGCCCATCG GGTAATAACC AAAACCAAGG CAACATGCAG
1081     AGGGAGCCAA ACCAGGCCTT CGGTTCTGGA AATAACTCTT ATAGTGGCTC TAATTCTGGT
1141     GCAGCAATTG GTTGGGGATC AGCATCCAAT GCAGGGTCGG GCAGTGGTTT TAATGGAGGC
1201     TTTGGCTCAA GCATGGATTC TAAGTCTTCT GGCTGGGGAA TGTAG

 

Quality Control:cDNA sequencing results is available upon request.