Anti-Syndecan-1 Antibody

Cat: 11429-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All Syndecan-1 Reagents
CRO Services
Product recommended

All Syndecan-1 Reagents
Anti-Syndecan-1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Syndecan-1 Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Syndecan-1
Immunogen
Recombinant Human Syndecan-1 / SDC1 / CD138 protein (Catalog#11429-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Syndecan-1 / SDC1 / CD138 (rh Syndecan-1 / SDC1 / CD138; Catalog#11429-H08H; NP_002988.3; Met 1-Glu 251). Syndecan-1 / SDC1 / CD138 specific IgG was purified by Human Syndecan-1 / SDC1 / CD138 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Syndecan-1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Syndecan-1 Antibody (Rabbit Polyclonal antibody) Images
Human Syndecan-1/CD138/SDC1 Immunohistochemistry(IHC) 3628
Immunochemical staining of human SDC1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human Syndecan-1/CD138/SDC1 Immunohistochemistry(IHC) 3629
Immunochemical staining of human SDC1 in human colon cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.
Human Syndecan-1/CD138/SDC1 Immunohistochemistry(IHC) 3630
Immunochemical staining of human SDC1 in human breast cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.
Human Syndecan-1/CD138/SDC1 Western blot (WB) 13403

Anti-SDC1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: MCF-7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:55 kDa

Anti-Syndecan-1 Antibody Alternative Names
Anti-CD138 Antibody;Anti-SDC Antibody;Anti-SYND1 Antibody;Anti-syndecan Antibody;Anti-Syndecan-1 Antibody
Syndecan-1 Background Information

Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.

Full Name
syndecan 1
References
  • Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.
  • Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.
  • Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA 80344-T16
    Anti-Syndecan-1 Antibody Rabbit Monoclonal ELISA 50641-R004
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA 50641-T16
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA 50641-RP01
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA,IHC-P 50641-RP02
    Anti-Syndecan-1 Antibody (APC) Rabbit Monoclonal FCM 11429-R017-A
    Anti-Syndecan-1 Antibody Rabbit Polyclonal WB,IP 100620-T36
    Anti-Syndecan-1 Antibody (FITC) Rabbit Monoclonal FCM 11429-R017-F
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA 11429-RP01
    Anti-Syndecan-1 Antibody (PE) Rabbit Monoclonal FCM 11429-R017-P
    Anti-Syndecan-1 Antibody Rabbit Monoclonal ELISA 11429-R001
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA 11429-T16
    Anti-Syndecan-1 Antibody Rabbit Polyclonal ELISA,IHC-P 11429-T24
    Anti-Syndecan-1 Antibody Rabbit Monoclonal FCM 11429-R017
    info info
    添加购物车成功! 添加购物车失败!