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Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified

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SDC1Antibody Product Information
Antigen:Recombinant Human Syndecan-1 / SDC1 / CD138 protein (Catalog#11429-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Syndecan-1 / SDC1 / CD138 (rh Syndecan-1 / SDC1 / CD138; Catalog#11429-H08H; NP_002988.3; Met 1-Glu 251). Syndecan-1 / SDC1 / CD138 specific IgG was purified by Human Syndecan-1 / SDC1 / CD138 affinity chromatography.
SDC1Antibody Usage Guide
Specificity:Human Syndecan-1 / SDC1 / CD138
Application:WB, IHC-P

WB: 2-5 μg/mL

IHC-P: 0.1-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunochemistry

Immunochemical staining of human SDC1 in human breast cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.

Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunochemistry

Immunochemical staining of human SDC1 in human colon cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.

Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunochemistry

Immunochemical staining of human SDC1 in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

Background

Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.

References
  • Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.
  • Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.
  • Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
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