SynCam / CADM1 / TSLC1 / IGSF4 Antibody (Antigen Affinity Purified)

SynCam / CADM1 Antibody (Antigen Affinity Purified) Datasheet

SynCam / CADM1 Antibody Product Information

• Immunogen: Recombinant Human SynCam / CADM1 protein (Catalog#11168-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human SynCam / CADM1 (rh SynCam / CADM1; Catalog#11168-H08H; NP_055148.3; Met 1- His 374). SynCam / CADM1 specific IgG was purified by Human SynCam / CADM1 affinity chromatography.

SynCam / CADM1 Antibody Usage Guide

• Specificity: Human SynCam / CADM1
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CADM1 in WB. Using a DAB detection system, the detection limit for Human CADM1 is approximately 4 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CADM1. The detection limit for Human CADM1 is 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SynCam / CADM1 Antibody Background

Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 1, also known as Immunoglobulin superfamily member 4, Nectin-like protein 2, CADM1, IGSF4, and IGSF4A, is a single-pass type I  membrane protein which belongs to the nectin family. CADM1 contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CADM1 mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. CADM1 may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

References

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