|IP||0.5-2 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Sox2 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate2 µL anti-Sox2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-Sox2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 37 kDa
Observed band size: 41 kDa
Anti-Sox2 rabbit polyclonal antibody at 1:500 dilution
Lane A: HESS9 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:34 kDa
Observed band size:37 kDa
(We are unsure as to the identity of these extra bands.)
SOX2, also known as ANOP3, is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. SOX2 is critical for early embryogenesis and for embryonic stem cell pluripotency. It keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Defects in SOX2 can cause microphthalmia, syndromic, 3. A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.