Mouse Smad3 Gene cDNA Clone / ORF Clone

SMAD3 cDNA Clone Datasheet

SMAD3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_016769.4
• cDNA Size: 1278
• Gene Synonym: Madh3; AU022421; Smad3
• Source: Mouse

SMAD3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus MAD homolog 3 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SMAD3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SMAD3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SMAD3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGTCCA TCCTGCCCTT CACCCCCCCG ATCGTGAAGC GCCTGCTGGG TTGGAAGAAG
61       GGCGAGCAGA ACGGGCAGGA GGAGAAGTGG TGCGAGAAGG CGGTCAAGAG CTTGGTGAAG
121      AAGCTCAAGA AGACGGGGCA GTTGGACGAG CTGGAGAAGG CCATCACCAC GCAGAACGTG
181      AACACCAAGT GCATTACCAT CCCCAGGTCA CTGGATGGTC GGCTGCAGGT GTCCCATCGG
241      AAGGGGCTCC CTCACGTTAT CTACTGCCGC CTGTGGCGAT GGCCCGACCT GCACAGCCAC
301      CATGAATTAC GGGCCATGGA GCTCTGTGAG TTTGCCTTCA ACATGAAGAA GGATGAAGTG
361      TGTGTAAATC CTTACCACTA TCAGAGAGTA GAGACGCCAG TTCTACCTCC AGTGTTGGTG
421      CCACGCCACA CCGAGATCCC GGCCGAGTTC CCCCCACTGG ATGACTACAG CCATTCCATT
481      CCCGAGAACA CTAACTTCCC TGCTGGCATT GAGCCCCAGA GCAATATTCC AGAAACCCCA
541      CCTCCTGGCT ACCTGAGTGA AGATGGAGAA ACCAGTGACC ACCAGATGAA CCACAGCATG
601      GACGCAGGTT CTCCAAACCT CTCCCCGAAT CCGATGTCCC CAGCACACAA TAACTTGGAC
661      CTACAGCCAG TCACCTACTG TGAGCCGGCC TTCTGGTGCT CCATCTCCTA CTACGAGCTG
721      AACCAGCGAG TTGGGGAGAC ATTCCACGCC TCACAGCCAT CCATGACAGT AGATGGCTTC
781      ACTGACCCCT CCAACTCGGA GCGCTTCTGC CTGGGCCTAC TGTCCAATGT CAACCGGAAT
841      GCAGCCGTGG AACTTACAAG GCGACACATT GGGAGAGGTG TGCGGCTCTA CTACATCGGA
901      GGGGAGGTCT TTGCGGAGTG CCTCAGTGAC AGTGCTATTT TCGTCCAGTC TCCCAACTGC
961      AACCAGCGCT ATGGCTGGCA CCCGGCCACT GTCTGCAAGA TCCCACCAGG CTGCAACCTG
1021     AAGATCTTCA ACAACCAGGA ATTTGCTGCC CTCCTAGCTC AGTCTGTCAA CCAGGGCTTT
1081     GAGGCTGTCT ACCAGCTGAC GCGCATGTGC ACCATCCGTA TGAGCTTCGT CAAAGGCTGG
1141     GGAGCAGAGT ACAGGAGACA GACAGTGACC AGCACCCCCT GCTGGATTGA GCTACACCTG
1201     AATGGACCCT TGCAGTGGCT TGACAAGGTC CTCACCCAGA TGGGTTCCCC GAGCATCCGC
1261     TGTTCCAGTG TGTCTTAG

 

Quality Control:cDNA sequencing results is available upon request.