Mouse SERPINB6B Protein (His Tag)

Serine (or cysteine) peptidase inhibitor, clade B, member 6b Protein Datasheet

SERPINB6B Protein Product Information

• Synonym: RP23-391I11.3, NK13, Spi12, ovalbumin
• Protein Construction: A DNA sequence encoding the mouse SERPINB6B (NP_035584.1) (Met 1-Pro 377) was expressed，with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

SERPINB6B Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant mouse SERPINB6B consists of 387 amino acids and has a calculated molecular mass of 44 kDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH 7.0, 10%gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SERPINB6B protein

SERPINB6B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SERPINB6B Protein Description

SerpinB6, also known as Cytoplasmic antiproteinase, Peptidase inhibitor 6, Placental thrombin inhibitor, SERPINB6 and PI-6, is a cytoplasm protein which belongs to the?serpin family and Ov-serpin subfamily. SerpinB6 / PI-6 is an inhibitor of cathepsin G, kallikrein-8 and thrombin. It may play an important role in the inner ear in the protection against leakage of lysosomal content during stress and loss of this protection results in cell death and sensorineural hearing loss. SerpinB6 / PI-6 is expressed in keratinocytes (at protein level). It is also found in placenta, cardiac muscle, lung, liver, kidney and pancreas. SerpinB6 / PI-6 is expressed in the inner ear hair cells. It expressed abundantly by normal mast cells in different tissues and by mast cells in mastocytoma lesions. SerpinB6 / PI-6 may be involved in the regulation of serine proteinases present in the brain or extravasated from the blood. Defects in SerpinB6 are the cause of deafness autosomal recessive type 91 which is a form of non-syndromic deafness characterized by progressive and age-dependent sensorineural hearing loss. Vestibular function is normal. SerpinB6B is a member of SerpinB6 subfamily.

References

1. Morgenstern KA. et al.,1994, Biochemistry. 33: 3432-41.
2. Strik MC. et al., 2004, Blood. 103: 2710-7.
3. Scott FL. et al., 2007, J Biochem. 142: 435-42.
4. Burkard TR. et al., 2011, BMC Syst Biol. 5: 17.