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Mouse SERPINA1B Gene cDNA clone plasmid

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SERPINA1BcDNA Clone Product Information
Gene_bank_ref_id:NM_009244.4
RefSeq ORF Size:1242bp
cDNA Description:Full length Clone DNA of Mus musculus serine (or cysteine) preptidase inhibitor, clade A, member 1b.
Gene Synonym:PI2, Dom2, Spi1-2, D12Ucla2, Serpina1b
Species:Mouse
Vector:pMD18-T Simple Vector
Plasmid:pMD-mSERPINA1B
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for ten point mutations: 52 A/C resulting in the amino acid Met substitution by Leu, 719 720 TG/CC resulting in the amino acid Met substitution by Thr, 755 T/C resulting in the amino acid ILe substitution by Thr, 57 T/C, 156 C/A, 388 C/T, 666 G/A, 759 G/C and 1011 T/C not causing the amino acid variation.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Other SERPINA1B Protein Products
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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