SerpinI1 Antibody (Antigen Affinity Purified)

SerpinI1 Antibody ( Antigen Affinity Purified ) Datasheet

SerpinI1 Antibody Product Information

• Immunogen: Recombinant Human SerpinI1 protein (Catalog#11107-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human SerpinI1 (rh SerpinI1; Catalog#11107-H08H; NP_005016.1; Met 1-Leu 410). SerpinI1 specific IgG was purified by Human SerpinI1 affinity chromatography.

SerpinI1 Antibody Usage Guide

• Specificity: Human SerpinI1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human SerpinI1 in human brain with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SerpinI1. The detection limit for Human SerpinI1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinI1 Antibody Background

Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein which belongs to theserpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).

References

1. Schrimpf, SP. et al., 1997, Genomics. 40 (1): 55-62.
2. Hill, RM.et al., 2002,Ann N Y Acad Sci. 971: 406-15.
3. Yepes, M. et al., 2004, Thromb. Haemost. 91 (3): 457-64.
4. Galliciotti, G. et al., 2006, Front Biosci. 11: 33-45.