|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
SERPINF2 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate2 µL anti-SERPINF2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-SERPINF2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 55 kDa
Observed band size: 66 kDa
Anti-SerpinF2 rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:55 kDa
Observed band size:55 kDa
(We are unsure as to the identity of these extra bands.)
SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Polyclonal||ELISA||50167-RP02|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Polyclonal||ELISA||50167-RP01|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Monoclonal||ELISA,ELISA(Cap),IHC-P||50167-R128|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Polyclonal||WB,ELISA||10297-RP01|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Monoclonal||ELISA(Det)||10297-R025|
|Anti-Alpha 2 Antiplasmin Antibody||Mouse||Monoclonal||ELISA||10297-MM05|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Monoclonal||WB,ELISA,IP||10297-R001|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Monoclonal||ELISA,IHC-P||10297-R043|
|Anti-Alpha 2 Antiplasmin Antibody (FITC)||Rabbit||Monoclonal||FCM||10297-R034-F|
|Anti-Alpha 2 Antiplasmin Antibody (PE)||Rabbit||Monoclonal||FCM||10297-R034-P|
|Anti-Alpha 2 Antiplasmin Antibody (FITC)||Mouse||Monoclonal||FCM||10297-MM08-F|
|Anti-Alpha 2 Antiplasmin Antibody||Rabbit||Monoclonal||ELISA(Cap)||10297-R007|
|Anti-Alpha 2 Antiplasmin Antibody||Mouse||Monoclonal||FCM||10297-MM08|