SerpinF2 / AAP Antibody (Antigen Affinity Purified)

Alpha-2 Antiplasmin Antibody ( Antigen Affinity Purified )

SerpinF2 / AAP Antibody Product Information

• Immunogen: Recombinant human SerpinF2 protein ( Catalog#10297-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human SerpinF2 ( rh SerpinF2 ; Catalog#10297-H08H ; NP_000925.2 ; Met 1 - Lys 491 ). SerpinF2 specific IgG was purified by human SerpinF2 affinity chromatography.

SerpinF2 / AAP Antibody Usage Guide

• Specificity: Human SerpinF2 / AAP
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SerpinF2 in WB. Using a DAB detection system, the detection limit for human SerpinF2 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human SerpinF2. The detection limit for human SerpinF2 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinF2 / AAP Antibody Background

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed at moderate level in other tissues such as muscle, intestine, central nervous system and placenta. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the deficiency of Alpha-2 antiplasmin in humans results in uncontrolled fibrinolysis and a bleeding disorder.

References

1. Potempa, J. et al., 1988, Science. 241: 699-700.
2. Menoud, P.A. et al., 1996, J. Clin. Invest. 97: 2478-2484.
3. Mutch, NJ. et al., 2007, J. Thromb. Haemost. 5: 812-817.
4. Victoria, J. et al., 2007, Blood, 109: 5286-5292.