Anti-Alpha 2 Antiplasmin Antibody

Cat: 10297-RP02
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All Alpha 2 Antiplasmin Reagents
Anti-Alpha 2 Antiplasmin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Alpha 2 Antiplasmin Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human Alpha 2 Antiplasmin
Immunogen
Recombinant Human SerpinF2 protein (Catalog#10297-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinF2 (rh SerpinF2; Catalog#10297-H08H; NP_000925.2; Met 1-Lys 491). SerpinF2 specific IgG was purified by human SerpinF2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Alpha 2 Antiplasmin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IHC-P 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Alpha 2 Antiplasmin Antibody (Rabbit Polyclonal antibody) Images
Human SerpinF2 Immunohistochemistry(IHC) 3614
Immunochemical staining of human SerpinF2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human SerpinF2 Immunoprecipitation(IP) 7968

SERPINF2 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SERPINF2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SERPINF2 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 66 kDa

Human SerpinF2 Western blot (WB) 13174

Anti-SerpinF2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Alpha 2 Antiplasmin Antibody Alternative Names
Anti-A2AP Antibody;Anti-AAP Antibody;Anti-ALPHA-2-PI Antibody;Anti-API Antibody;Anti-PLI Antibody
Alpha 2 Antiplasmin Background Information

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

Full Name
serpin peptidase inhibitor, clade F, member 2
References
  • Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.
  • Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.
  • Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.
  • Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Polyclonal ELISA 50167-RP02
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Polyclonal ELISA 50167-RP01
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Monoclonal ELISA,ELISA(Cap),IHC-P 50167-R128
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Polyclonal WB,ELISA 10297-RP01
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Monoclonal ELISA(Det) 10297-R025
    Anti-Alpha 2 Antiplasmin Antibody Mouse Monoclonal ELISA 10297-MM05
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Monoclonal WB,ELISA,IP 10297-R001
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Monoclonal ELISA,IHC-P 10297-R043
    Anti-Alpha 2 Antiplasmin Antibody (FITC) Rabbit Monoclonal FCM 10297-R034-F
    Anti-Alpha 2 Antiplasmin Antibody (PE) Rabbit Monoclonal FCM 10297-R034-P
    Anti-Alpha 2 Antiplasmin Antibody (FITC) Mouse Monoclonal FCM 10297-MM08-F
    Anti-Alpha 2 Antiplasmin Antibody Rabbit Monoclonal ELISA(Cap) 10297-R007
    Anti-Alpha 2 Antiplasmin Antibody Mouse Monoclonal FCM 10297-MM08
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