SerpinD1 Protein (His Tag)

Serpin Peptidase Inhibitor Clade D Member 1 Protein

SerpinD1 / HC2 Protein Product Information

• SerpinD1 Synonym: SerpinD1, HC2, LS2, HCF2, HCII, HLS2, D22S673
• Protein Construction: A DNA sequence encoding the human SerpinD1 precursor ( NP_000176.2 ) ( Met 1 - Ser 499 ) was expressed with a C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

SerpinD1 / HC2 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 20
• Molecular Mass: The mature recombinant human SerpinD1 consists of 491 amino acids and predicts a molecular mass of 56.4 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhSerpinD1 is approximately 65-70 KDa as a result of glycosylation.
• Formulation: Lyophilized from sterile PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SerpinD1 protein

SerpinD1 / HC2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SerpinD1 / HC2 Protein Description

SerpinD1, also known as heparin cofactor II (HCII),is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis.

References

1. Van Deerlin, V. M. et al., 1991, J. Biol. Chem. 266: 20223-20231
2. Giri, T.K. et al., 2006, Blood. 107: 2753-2758.
3. Bauman, S.J. et al., 1999, J. Biol. Chem. 274: 34556-34565.
4. Tollefsen, D.M. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27: 454-460.