Mouse SerpinD1 / HCII Protein (His Tag)

Heparin cofactor 2 ( SerpinD1 / HCII ) Protein Datasheet

SerpinD1 / HCII Protein Product Information

• Synonym: Serpind1, AA985900 , AI30344 6, HCII , Hcf2 , MGC107662
• Protein Construction: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

SerpinD1 / HCII Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit thombin ( Sigma, T4648 ) cleavage of fluorogenic peptide substrate Boc-VPR-AMC ( R&D Systems, Catslog# ES011).
  The IC₅₀ value is < 1.2 nM
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 24
• Molecular Mass: The recombinant mouse SerpinD1 consists of 466 amino acids and predicts a molecular mass of 53.5 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmSerpinD1 is approximately 65-70 KDa as a result of glycosylation
• Formulation: Lyophilized from sterile 25mM HEPES, 150mM NaCl, pH 7.4
• SDS-PAGE:

SerpinD1 / HCII Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SerpinD1 / HCII Protein Description

SerpinD1, also known as heparin cofactor II (HCII), is a member of Serpin superfamily of the serine proteinase inhibitors. Mouse HCII is a glycoprotein expressed predominantly in liver that inhibits thrombin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans (GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis.

References

1. Zhang, G.S. et al., 1994, Biochemistry. 33: 3632-42.
2. Giri, T.K. et al., 2006, Blood. 107: 2753-8.
3. Bauman, S.J. et al., 1999, J. Biol. Chem. 274: 34556-65.
4. Tollefsen, DM. et al., 2007, Arterioscler.Thromb.Vasc.Biol. 27: 454-60.