SerpinD1 / HCII Antibody (Antigen Affinity Purified)

Mouse Heparin Cofactor II Antibody ( Antigen Affinity Purified )

SerpinD1 / HCII Antibody Product Information

• Immunogen: Recombinant mouse SerpinD1 protein ( Catalog#50121-M08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse SerpinD1 ( rM SerpinD1 ; Catalog#50121-M08H ; NP_032249.3 ; Met 1 - Ser 478 ). SerpinD1 specific IgG was purified by mouse SerpinD1 affinity chromatography.

SerpinD1 / HCII Antibody Usage Guide

• Specificity: Mouse SerpinD1 / HCII
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse SerpinD1 in WB. Using a DAB detection system, the detection limit for mouse SerpinD1 is approximately 0.5 ng/lane under non-reducing conditions and reduced conditions.
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect mouse SerpinD1. The detection limit for mouse SerpinD1 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinD1 / HCII Antibody Background

Mouse SerpinD1, also known as heparin cofactor II (HCII), is a member of Serpin superfamily of the serine proteinase inhibitors. Mouse HCII is a glycoprotein expressed predominantly in liver that inhibits thrombin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans (GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis.

References

1. Zhang, G.S. et al., 1994, Biochemistry. 33: 3632-42.
2. Giri, T.K. et al., 2006, Blood. 107: 2753-8.
3. Bauman, S.J. et al., 1999, J. Biol. Chem. 274: 34556-65.
4. Tollefsen, DM. et al., 2007, Arterioscler.Thromb.Vasc.Biol. 27: 454-60.