Anti-SerpinD1 Antibody

Cat: 10295-RP02
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Anti-SerpinD1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SerpinD1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SerpinD1
Immunogen
Recombinant Human SerpinD1 protein (Catalog#10295-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinD1 (rh SerpinD1; Catalog#10295-H08H; NP_000176.2; Met 1-Ser 499). SerpinD1 specific IgG was purified by human SerpinD1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SerpinD1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SerpinD1 Antibody (Rabbit Polyclonal antibody) Images
Human SerpinD1 Immunoprecipitation(IP) 7966

SerpinD1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SerpinD1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SerpinD1 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 70 kDa

Human SerpinD1 Western blot (WB) 13171

Anti-SerpinD1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:70 kDa

Anti-SerpinD1 Antibody Alternative Names
Anti-D22S673 Antibody;Anti-HC2 Antibody;Anti-HCF2 Antibody;Anti-HCII Antibody;Anti-HLS2 Antibody;Anti-LS2 Antibody;Anti-SerpinD1 Antibody;Anti-THPH10 Antibody
SerpinD1 Background Information

SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Full Name
serpin peptidase inhibitor, clade D (heparin cofactor), member 1
References
  • Rau JC, et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study. Exp Mol Pathol. 87(3): 178-83.
  • Aihara K, et al. (2009) Heparin cofactor II as a novel vascular protective factor against atherosclerosis. J Atheroscler Thromb. 16(5): 523-31.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SerpinD1 Antibody Rabbit Polyclonal ELISA 50121-RP01
    Anti-SerpinD1 Antibody Rabbit Polyclonal ELISA,IHC-P 50121-RP02
    Anti-SerpinD1 Antibody Rabbit Monoclonal WB,ELISA,IP 50121-R025
    Anti-SerpinD1 Antibody Rabbit Polyclonal WB,ELISA 10295-RP01
    Anti-SerpinD1 Antibody Mouse Monoclonal ELISA 10295-MM02
    Anti-SerpinD1 Antibody Mouse Monoclonal IHC-P 10295-MM07
    Anti-SerpinD1 Antibody Rabbit Monoclonal IHC-P 10295-R008
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