Anti-SerpinB8 Antibody

Cat: 50215-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All SerpinB8 Reagents
CRO Services
Product recommended

All SerpinB8 Reagents
Anti-SerpinB8 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SerpinB8 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse SerpinB8
Immunogen
Recombinant Mouse SerpinB8 protein (Catalog#50215-M08B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SerpinB8 (rM SerpinB8; Catalog#50215-M08B; NP_035589.1; Met 1-Pro 374). SerpinB8 specific IgG was purified by Mouse SerpinB8 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SerpinB8 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SerpinB8 Antibody (Rabbit Polyclonal antibody) Images
Mouse SerpinB8 Immunoprecipitation(IP) 6089

Mouse SERPINB8 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-Mouse SERPINB8 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse SERPINB8 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 43 kDa

Mouse SerpinB8 Western blot (WB) 17497

Anti-SERPINB8 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:43 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SerpinB8 Antibody Alternative Names
Anti-CAP-2 Antibody;Anti-CAP2 Antibody;Anti-NK10 Antibody;Anti-ovalbumin Antibody;Anti-Spi8 Antibody
SerpinB8 Background Information

Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional diagnostic immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.

Full Name
serpin peptidase inhibitor, clade B (ovalbumin), member 8
References
  • Sumi, Y. et al., 1989, J. Biochem. 106: 703-7.
  • Rawlings, N.D. et al., 2004, Biochem J. 378: 705-16.
  • Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42.
  • Filleur, S. et al., 2009, J Cell Biochem. 106 (5): 769-75.
  • de Koning, P.J. et al., 2009, Pancreas. 38 (4): 461-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SerpinB8 Antibody Rabbit Monoclonal ELISA 50215-R013
    Anti-SerpinB8 Antibody Rabbit Polyclonal ELISA 50215-RP01
    info info
    添加购物车成功! 添加购物车失败!