SERPINB2 / SerpinB2 Protein (GST Tag)

Plasminogen Activator Inhibitor 2 Protein

SERPINB2 / SerpinB2 Protein Product Information

• Synonym: HsT1201, PAI, PAI-2, PAI2, PLANH2
• Protein Construction: A DNA sequence encoding the full length of human SERPINB2 (NP_002566.1) ( Met 1 - Pro 415 ) was fused with the GST tag at the N-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

SERPINB2 / SerpinB2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human SERPINB2/GST chimera consists of 640 amino acids and predicts a molecular mass of 73 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris,100mM NaCl, pH 8.0 , 0.5 mM Reduced Glutathione, 10% glycerol, 0.5mM PMSF
• SDS-PAGE: SerpinB2 protein

SERPINB2 / SerpinB2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SERPINB2 / SerpinB2 Protein Description

Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
SerpinB2, also known as Plasminogen activator inhibitor 2, Placental plasminogen activator inhibitor, Monocyte Arg-serpin, Urokinase inhibitor and PAI2, is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. SerpinB2 is a major product of activated monocytes and macrophages and is substantially induced during most inflammatory processes. Distinct from its widely described extracellular role as an inhibitor of urokinase plasminogen activator. SerpinB2 has been shown to have an intracellular role as a retinoblastoma protein (Rb)-binding protein that inhibits Rb degradation. SerpinB2 is widely described as an inhibitor of urokinase plasminogen activator. SerpinB2 inhibits urokinase-type plasminogen activator. The monocyte derived SerpinB2 is distinct from the endothelial cell-derived PAI-1. SerpinB2 is a potentially important inducible host factor that significantly promotes HIV-1 replication.

References

1. Sumi, Y. et al., 1989, J. Biochem. 106: 703-7.
2. Rawlings, N.D. et al., 2004, Biochem J. 378: 705-16.
3. Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42.
4. Schroder,WA. et al., 2010, J Immunol. 184 (5): 2663-70.