Mouse SERPINB12 / SerpinB12 Protein (His Tag)

Mouse SerpinB12 / SERPINB12 Protein Datasheet

SerpinB12 / SERPINB12 Protein Product Information

• Synonym: 2300003F07Rik, 4833409F13Rik
• Protein Construction: A DNA sequence encoding the mouse SERPINB12 ( NP_082247.1 ) ( Met 1 - Pro 423 ) was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

SerpinB12 / SERPINB12 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant mouse SERPINB12 consists of 434 amino acids and has a calculated molecular mass of 49.3 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris,100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 7.4
• SDS-PAGE: SerpinB12 protein

SerpinB12 / SERPINB12 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SerpinB12 / SERPINB12 Protein Description

Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Most serpins are secreted and attain physiologic concentrations in the blood and extracellular fluids. Mouse SerpinB12 is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. It is expressed in many tissues, including brain, bone marrow, lymph node, heart, lung, liver, pancreas, testis, ovary, and intestine. SerpinB12 inhibits trypsin and plasmin, but not thrombin, coagulation factor Xa, or urokinase-type plasminogen activator.

References

1. Askew,Y.S. et al., 2001, J Biol Chem. 276 (52):49320-30.
2. Rawlings, ND. et al., 2004, Biochem J. 378: 705-16.
3. Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42.
4. Filleur, S. et al., 2009, J Cell Biochem. 106 (5): 769-75.
5. de Koning, PJ. et al., 2009, Pancreas. 38 (4): 461-7.