SerpinA7 / TBG Protein (His Tag)

Thyroxine-binding globulin ( SerpinA7 / TBG ) Protein Datasheet

SerpinA7 / TBG Protein Product Information

• Synonym: SERPINA7 , RP1-82J11.2, TBG
• Protein Construction: A DNA sequence encoding the human SERPINA7 (NP_000345.2 ) (Met 1-Ala 415) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

SerpinA7 / TBG Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 21
• Molecular Mass: The secreted recombinant human SERPINA7 comprises 406 amino acids and has a predicted molecular mass of 45.5 kDa. As a result of glycosylation, it migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS, pH7.4
• SDS-PAGE:

SerpinA7 / TBG Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SerpinA7 / TBG Protein Description

Thyroxine-binding globulin, also known as T4-binding globulin, SerpinA7 and TBG, is a secreted protein which belongs to the serpin family. TBG is synthesized primarily in the liver and secreted in plasma. Genomically, TBG is a serpin, although it has no inhibitory function like many other members of this class of proteins. TBG binds thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin ) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3’-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 and T3. TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG tests are sometimes used in finding the reason for elevated or diminished levels of thyroid hormone, and defects in SerpinA7 are a cause of TBG deficiency.

References

1. Cheng, SY.et al.,1978,Biochem. Biophys.Res.Commun. 79 (4): 1212-8.
2. Bertenshaw, R. et al.,1991, Am. J. Hum. Genet. 48 (4): 741-4.
3. Imamura, S. et al., 1991, Biochemistry. 30 (22): 5406-11.
4. Bertenshaw, R. et al., 1992, Biochim. Biophys. Acta 1139 (4): 307-10.
5. Shirotani, T. et al., 1993, Endocrinol. Jpn. 39 (6): 577-84.