SerpinA7 Antibody

SerpinA7 Antibody Datasheet

SerpinA7 Antibody Product Information

• Immunogen: Recombinant Mouse SerpinA7 Protein (Catalog#50313-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse SerpinA7 (rM SerpinA7; Catalog#50313-M08H; NP_808588.3; Met 1- Gln 418).

SerpinA7 Antibody Usage Guide

• Specificity: Mouse SerpinA7
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse SERPINA7 in WB. Using a DAB detection system, the detection limit for Mouse SERPINA7 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SERPINA7. The detection limit for Mouse SERPINA7 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinA7 Antibody Background

Thyroxine-binding globulin, also known as T4-binding globulin, Serpin A7 and TBG, is a secreted protein which belongs to the serpin family. TBG is synthesized primarily in the liver as a 54 kDa protein.TBG is genomically a serpin, although it has no inhibitory function like many other members of this class of proteins. TBG binds thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3’-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 and T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG tests are sometimes used in finding the reason for elevated or diminished levels of thyroid hormone.

References

1. Cheng, SY. et al., 1978, Biochem. Biophys. Res. Commun. 79 (4): 1212-8.
2. Bertenshaw, R. et al.,1991, Am. J. Hum. Genet. 48 (4): 741-4.
3. Imamura, S. et al., 1991, Biochemistry. 30 (22): 5406-11.
4. Bertenshaw, R. et al., 1992, Biochim. Biophys. Acta 1139 (4): 307-10.
5. Shirotani, T. et al., 1993, Endocrinol. Jpn. 39 (6): 577-84.