SerpinA6 Antibody (Antigen Affinity Purified)

SerpinA6 Antibody ( Antigen Affinity Purified ) Datasheet

SerpinA6 Antibody Product Information

SerpinA6 Antibody Usage Guide

Immunogen :	Recombinant Mouse SerpinA6 protein (Catalog#50314-M08H)
Antibody Type :	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type :	Rabbit IgG
Formulation :	0.2 μm filtered solution in PBS with 5% trehalose
Preparation :	Produced in rabbits immunized with purified, recombinant Mouse SerpinA6 (rM SerpinA6; Catalog#50314-M08H; Q06770; Met 1-Ala 397). SerpinA6 specific IgG was purified by Mouse SerpinA6 affinity chromatography.

• Specificity: Mouse SerpinA6
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SERPINA6 in WB. Using a DAB detection system, the detection limit for Mouse SERPINA6 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SERPINA6. The detection limit for Mouse SERPINA6 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinA6 Antibody Background

The serpin peptidase inhibitor, clade A, member 6 (SerpinA6), also known as corticosteroid-binding globulin or CBG, is a member of Serpin (Serine Protease Inhibitors) superfamily. The superfamily of serine protease inhibitors (SERPINs) plays a key role in controlling the activity of proteinases in diverse biological processes. SerpinA6 is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and SerpinA6, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to SerpinA6 in human plasma). In this context, SerpinA6 directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. As a specific high-affinity plasma transport glycoprotein for cortisol. Stress-induced falls in CBG levels may heighten hypothalamic-pituitary-adrenal axis responses and CBG. The tissue interactions may allow targeted cortisol delivery. Three genetic variants of CBG have been identified that reduce cortisol binding affinity and/or CBG levels. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. SerpinA6/CBG is the major transport protein for glucocorticoids and progestins in the blood of most vertebrates, and may involved in obesity and stress sensitivity.

References

1. Gagliardi L. et al., 2010, Mol Cell Endocrinol.316 (1): 24-34.
2. Seralini GE. 1991, C R Seances Soc Biol Fil. 185 (6): 500-9.
3. Seixas S. et al., 2007, Mol Biol Evol. 24 (2): 587-98.
4. Buss C. et al., 2007, J Neural Transm. 114 (5): 563-9.