SerpinA6 Antibody

SerpinA6 Antibody Datasheet

SerpinA6 Antibody Product Information

• Immunogen: Recombinant Human SerpinA6 protein (Catalog#10998-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 037
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human SerpinA6 (rh SerpinA6; Catalog#10998-H08H; NP_001747.2; Met 1-Val 405).

SerpinA6 Antibody Usage Guide

• Specificity: Human SerpinA6
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SerpinA6. The detection limit for Human SerpinA6 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinA6 Antibody Background

The serpin peptidase inhibitor, clade A, member 6 (SerpinA6), also known as corticosteroid-binding globulin or CBG, is a member of Serpin (Serine Protease Inhibitors) superfamily. The superfamily of serine protease inhibitors (SERPINs) plays a key role in controlling the activity of proteinases in diverse biological processes. SerpinA6 is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and SerpinA6, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to SerpinA6 in human plasma). In this context, SerpinA6 directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. As a specific high-affinity plasma transport glycoprotein for cortisol. Stress-induced falls in CBG levels may heighten hypothalamic-pituitary-adrenal axis responses and CBG. The tissue interactions may allow targeted cortisol delivery. Three genetic variants of CBG have been identified that reduce cortisol binding affinity and/or CBG levels. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. SerpinA6/CBG is the major transport protein for glucocorticoids and progestins in the blood of most vertebrates, and may involved in obesity and stress sensitivity.

References

1. Gagliardi L. et al., 2010, Mol Cell Endocrinol.316 (1): 24-34.
2. Seralini GE. 1991, C R Seances Soc Biol Fil. 185 (6): 500-9.
3. Seixas S. et al., 2007, Mol Biol Evol. 24 (2): 587-98.
4. Buss C. et al., 2007, J Neural Transm. 114 (5): 563-9.