Mouse SERPINA11 Gene cDNA Clone / ORF Clone

SERPINA11 cDNA Clone Datasheet

SERPINA11 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_199314.2
• cDNA Size: 1275
• Gene Synonym: Gm895; MGC37881; Serpina11
• Source: Mouse

SERPINA11 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 11 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for six point mutations:425 T/C resulting in the amino acid Phe substitution by Ser, 657 C/G resulting in the amino acid Cys substitution by Trp, and 126 C/T, 237 T/C, 651C/A, 696 G/A not causing the amino acid variation.

SERPINA11 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SERPINA11 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SERPINA11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCCAG TGTGGCTGTG GCTGTGGCTG CTGGTAGCCG AGGTCCTGCT TCCTGTCCAT
61       TGTCAGCCAT TTTCTGCCCA TGGAGATAAG AGTCTGGGGG CATCTCAACC AGCCAGTCAC
121      CAGTCTCTGG AGCCAGCACC CGCCTATCAC AAGGTCACAC CCACCATTAC GAATTTTGCT
181      TTGCGTCTAT ACAAGCAACT AGCAGAGGAA GTAGCGGGAA ACATCCTTTT CTCCCCCGTG
241      AGCCTCTCCA GCAGCCTGGC TCTGCTGTCT CTTGGGGCAC ATGCTGACAC CCAGACTCAA
301      ATTCTGGAGA GCCTGGGCTT CAACCTCACA GAGACCCCAG CAGCTGACGT CCACCGGGGT
361      TTCCAGAGCC TCCTGCACAC TCTTGACCTC CCCAGCCCCA AACTGGAACT GAAACTGGGA
421      CATTCCCTGT TCCTAGACAG ACAGCTGAAG CCTCAGCAAC GTTTTCTGGA CAGTGCCAAG
481      GAACTGTATG GAGCTCTGGC CTTTTCTGCC AACTTCACAG AAGCAGCTGC CACAGGGCAG
541      CAGATCAATG ACCTAGTGAG GAAGCAGACC TACGGGCAGG TGGTGGGCTG TCTCCCGGAG
601      TTCAGCCATG ACACTCTCAT GGTTCTCTTG AACTACATCT TCTTCAAAGC AAAGTGGAAG
661      CATCCCTTTG ATCGCTACCA GACCCGGAAG CAGGAAAGCT TCTCCCTGGA CCAGAGGACG
721      CCACTCCGAA TTCCCATGAT GCGACAGAAG GAAATGCACA GGTTCCTGTA CGACCAGGAG
781      GCGTCGTGCA CTGTCCTCCA GATCGAGTAC AGTGGCACCG CCTTGCTGCT GCTGGTCCTC
841      CCTGACCCTG GGAAGATGCA GCAGGTAGAG GCTGCCCTCC AGCCAGAGAC GCTGAGAAGG
901      TGGGGCCAGC GGTTCCTGCC CAGTCTGTTG GATTTGCACC TGCCAAGGTT TTCAATTTCC
961      GCAACTTACA ACCTGGAAGA GATCCTGCCC CTCATTGGTC TTGGCAACCT ATTCGACATG
1021     GAAGCTGACT TATCAGGAAT TATGGGGCAA CTCAACAAAA CTGTCTCCAG GGTGTCACAC
1081     AAGGCCATCG TGGACATGAA CGAGAAGGGA ACTGAGGCTG CAGCTGCCTC TGGCCTCCTG
1141     TCCCAGCCCC CAGCCCTGAA CATGACATCA GCCCCACAGG CCCACTTTAA CAGGCCCTTC
1201     CTGCTTCTGC TCTGGGAGGT GACCACCCAG AGTCTGCTCT TCTTGGGGAA AGTGGTCAAC
1261     CCAGCTGCTG GGTAA

 

Quality Control:cDNA sequencing results is available upon request.