Anti-alpha 1 Antitrypsin Antibody

Cat: 10306-RP01
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Anti-alpha 1 Antitrypsin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-alpha 1 Antitrypsin Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human alpha 1 Antitrypsin
Immunogen
Recombinant Human SerpinA1 protein (Catalog#10306-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinA1 (rh SerpinA1; Catalog#10306-H08H; NP_000286.3; Met 1-Lys 418). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-alpha 1 Antitrypsin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-alpha 1 Antitrypsin Antibody (Rabbit Polyclonal antibody) Images
Human SerpinA1 Western blot (WB) 13177

Anti-SerpinA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:47 kDa

Observed band size:54 kDa

Anti-alpha 1 Antitrypsin Antibody Alternative Names
Anti-A1A Antibody;Anti-A1AT Antibody;Anti-AAT Antibody;Anti-alpha1AT Antibody;Anti-MGC23330 Antibody;Anti-MGC9222 Antibody;Anti-PI Antibody;Anti-PI1 Antibody;Anti-PRO2275 Antibody;Anti-SerpinA1 Antibody
alpha 1 Antitrypsin Background Information

SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. It association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and a complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.

Full Name
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
References
  • Khnlein T, et al. (2008) Alpha-1 antitrypsin deficiency: pathogenesis, clinical presentation, diagnosis, and treatment. Am J Med. 121(1): 3-9.
  • Camelier AA, et al. (2008) Alpha-1 antitrypsin deficiency: diagnosis and treatment. J Bras Pneumol. 34(7): 514-27.
  • Subramaniyam D, et al. (2010) Cholesterol rich lipid raft microdomains are gateway for acute phase protein, SERPINA1. Int J Biochem Cell Biol. 42(9): 1562-70.
  • Kilty SJ, et al. (2010) Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy. Am J Rhinol Allergy. 24(1): e4-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-alpha 1 Antitrypsin Antibody Rabbit Polyclonal WB,ELISA,IP 80476-T52
    Anti-alpha 1 Antitrypsin Antibody Rabbit Polyclonal ELISA 80476-RP01
    Anti-alpha 1 Antitrypsin Antibody Rabbit Polyclonal ELISA 80476-T16
    Anti-alpha 1 Antitrypsin Antibody Rabbit Monoclonal ELISA 80476-R005
    Anti-alpha 1 Antitrypsin Antibody Rabbit Polyclonal WB,IP 80476-T36
    Anti-alpha 1 Antitrypsin Antibody Rabbit Polyclonal ELISA 10306-RP02
    Anti-alpha 1 Antitrypsin Antibody Rabbit Monoclonal ELISA(Cap) 10306-R142
    Anti-alpha 1 Antitrypsin Antibody Rabbit Monoclonal ELISA(Det) 10306-R005
    Anti-alpha 1 Antitrypsin Antibody Rabbit Monoclonal ELISA 10306-R204
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