Mouse SEMA5A Gene cDNA Clone / ORF Clone

SEMA5A cDNA Clone Datasheet

SEMA5A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009154.2
• cDNA Size: 3224
• Gene Synonym: semF; Semaf; AI464145; 5930434A13; 9130201M22Rik; Sema5a
• Source: Mouse

SEMA5A cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 1503 C/T not causing the amino acid variation and the nucleotide 3209A deletion mutation.

SEMA5A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA5A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA5A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT
61       AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC
121      AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG
181      TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA
241      TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC
301      AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG
361      CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT
421      ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
481      TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT
541      GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT
601      TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG
661      TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT
721      GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG
781      CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG
841      CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG
901      GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT
961      GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC
1021     TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG
1081     GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT
1141     CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC
1201     CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT
1261     TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA
1321     GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG
1381     AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC
1441     AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG
1501     GATCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG
1561     AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT
1621     GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG
1681     GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG
1741     CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC
1801     TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA
1861     TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG
1921     GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA
1981     CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT
2041     GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA
2101     TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC
2161     AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA
2221     AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC
2281     AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT
2341     CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC
2401     AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC
2461     ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG
2521     GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC
2581     CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC
2641     CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA
2701     GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC
2761     ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT
2821     GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA
2881     AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT
2941     CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC
3001     CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC
3061     CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC
3121     AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
3181     TCCAACGCCT ACTTTACAGA TCTCAACATT ATGATGAATA CTAG

 

Quality Control:cDNA sequencing results is available upon request.