Semaphorin 4D / SEMA4D / CD100 Antibody (Antigen Affinity Purified)

Semaphorin 4D / SEMA4D / CD100 Antibody ( Antigen Affinity Purified ) Datasheet

Semaphorin 4D / SEMA4D / CD100 Antibody Product Information

• Immunogen: Recombinant Mouse Semaphorin 4D / SEMA4D / CD100 protein (Catalog#50642-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Semaphorin 4D / SEMA4D / CD100 (rM Semaphorin 4D / SEMA4D / CD100; Catalog#50642-M08H; NP_038688.2; Met 1-Arg 733). Semaphorin 4D / SEMA4D / CD100 specific IgG was purified by Mouse Semaphorin 4D / SEMA4D / CD100 affinity chromatography.

Semaphorin 4D / SEMA4D / CD100 Antibody Usage Guide

• Specificity: Mouse Semaphorin 4D / SEMA4D / CD100
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SEMA4D in WB. Using a DAB detection system, the detection limit for Mouse SEMA4D is approximately 1 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SEMA4D. The detection limit for Mouse SEMA4D is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Semaphorin 4D / SEMA4D / CD100 Antibody Background

Semaphorin-4D, also known as SEMA4D and CD100, is a single-pass type I membrane protein which belongs to thesemaphorin family. Semaphorin-4D / SEMA4D contains oneIg-like C2-type (immunoglobulin-like) domain, onePSI domain and oneSema domain. Semaphorin-4D / SEMA4D is strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. Semaphorin-4D / SEMA4D may play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability. Semaphorin-4D / SEMA4D is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin-B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system. In the immune system, Semaphorin-4D / SEMA4D binds CD72 to activate B cells and dendritic cells. It is involved in oligodendrogenesis during development and during recovery from ischemic injury.

References

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