Semaphorin 4A / SEMA4A / Semaphorin B Antibody (Antigen Affinity Purified)

Semaphorin 4A / SEMA4A / Semaphorin B Antibody ( Antigen Affinity Purified ) Datasheet

Semaphorin 4A / SEMA4A / Semaphorin B Antibody Product Information

• Immunogen: Recombinant Human Semaphorin 4A / SEMA4A / Semaphorin B protein (Catalog#11756-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Semaphorin 4A / SEMA4A / Semaphorin B (rh Semaphorin 4A / SEMA4A / Semaphorin B; Catalog#11756-H08H; NP_071762.2; Met 1-His 683). Semaphorin 4A / SEMA4A / Semaphorin B specific IgG was purified by Human Semaphorin 4A / SEMA4A / Semaphorin B affinity chromatography.

Semaphorin 4A / SEMA4A / Semaphorin B Antibody Usage Guide

• Specificity: Human Semaphorin 4A / SEMA4A / Semaphorin B
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SEMA4A in WB. Using a DAB detection system, the detection limit for Human SEMA4A is approximately 0.5 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SEMA4A. The detection limit for Human SEMA4A is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Semaphorin 4A / SEMA4A / Semaphorin B Antibody Background

Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I membrane protein which belongs to thesemaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A / SEMA4A contains oneIg-like C2-type (immunoglobulin-like) domain, onePSI domain and oneSema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 10 (CORD10) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.

References

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