Anti-SEMA4A Antibody

Cat: 11756-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All SEMA4A Reagents
CRO Services
Product recommended

All SEMA4A Reagents
Anti-SEMA4A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SEMA4A Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SEMA4A
Immunogen
Recombinant Human Semaphorin 4A / SEMA4A / Semaphorin B protein (Catalog#11756-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Semaphorin 4A / SEMA4A / Semaphorin B (rh Semaphorin 4A / SEMA4A / Semaphorin B; Catalog#11756-H08H; NP_071762.2; Met 1-His 683). Semaphorin 4A / SEMA4A / Semaphorin B specific IgG was purified by Human Semaphorin 4A / SEMA4A / Semaphorin B affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SEMA4A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SEMA4A Antibody (Rabbit Polyclonal antibody) Images
Human Semaphorin 4A/SEMA4A/Semaphorin B Immunoprecipitation(IP) 8108

SEMA4A was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-SEMA4A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SEMA4A rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 84 kDa

Observed band size: 105 kDa

Human Semaphorin 4A/SEMA4A/Semaphorin B Western blot (WB) 13447

Anti-SEMA4A rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:84 kDa

Observed band size:84 kDa

Anti-SEMA4A Antibody Alternative Names
Anti-CORD10 Antibody;Anti-RP35 Antibody;Anti-SEMAB Antibody;Anti-SEMB Antibody
SEMA4A Background Information

Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I  membrane protein which belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A / SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 10 (CORD10) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration.

Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.

Full Name
semaphorin 4A
References
  • Clark H.F., et al., 2003, Genome Res. 13: 2265-2270.
  • Ota T., et al., 2004,Nat. Genet. 36: 40-45.
  • Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60.
  • Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
  • Abid A., et al., 2006, J. Med. Genet. 43:378-381.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SEMA4A Antibody Mouse Monoclonal ELISA 11756-MM04
    info info
    添加购物车成功! 添加购物车失败!