|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human SUSD4 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13488-G-F|
|Human SUSD4 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13488-G-H|
|Human SUSD4 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13488-G-M|
|Human SUSD4 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13488-G-N|
|Human SUSD4 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13488-G-Y|
SUSD4, also known as sushi domain-containing protein 4, is a hypothetical cell surface protein whose tissue distribution and function are completely unknown. SUSD4 is detectable in murine brains, eyes, spinal cords, and testis but not other tissues. In brains, SUSD4 is highly expressed in the white matter on oligodendrocytes/axons, and in eyes, it is exclusively expressed on the photoreceptor outer segments. In in vitro complement assays, SUSD4 augments the alternative but not the classical pathway of complement activation at the C3 convertase step. SUSD4 deficiency may cause autism or Fryns syndrome, both of which are genetic diseases with severe abnormal neurological development and/or functions.