Anti-SUMO1 Antibody

Cat: 13095-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All SUMO1 Reagents
CRO Services
Product recommended

All SUMO1 Reagents
Anti-SUMO1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SUMO1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SUMO1
Immunogen
Recombinant Human SUMO1 / SUMO-1 protein (Catalog#13095-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human SUMO1 / SUMO-1 (rh SUMO1 / SUMO-1; Catalog#13095-H07E; AAH66306.1; Ser 2-Val 101). SUMO1 / SUMO-1 specific IgG was purified by Human SUMO1 / SUMO-1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SUMO1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:1000-1:5000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SUMO1 Antibody (Rabbit Polyclonal antibody) Images
Human SUMO1/SUMO-1 Immunoprecipitation(IP) 8155

sumo1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg A431 Whole Cell Lysate

2 µL anti-sumo1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-sumo1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 12 kDa

Observed band size: 18 kDa

Human SUMO1/SUMO-1 Western blot (WB) 13522

Anti-sumo1 rabbit polyclonal antibody at 1:1000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: A431 Whole Cell Lysate

Lane C: NIH-3T3 Whole Cell Lysate

Lane D: HL60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:15 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SUMO1 Antibody Alternative Names
Anti-DAP1 Antibody;Anti-GMP1 Antibody;Anti-OFC10 Antibody;Anti-PIC1 Antibody;Anti-SENP2 Antibody;Anti-SMT3 Antibody;Anti-SMT3C Antibody;Anti-SMT3H3 Antibody;Anti-UBL1 Antibody
SUMO1 Background Information

Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.

Full Name
small ubiquitin-like modifier 1
References
  • Kim HJ, et al. (2011) SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2. Biochem Biophys Res Commun. 410(3): 555-62.
  • Zuo Y, et al. (2009) Small ubiquitin-like modifier protein-specific protease 1 and prostate cancer. Asian J Androl. 11(1): 36-8.
  • Song T, et al. (2008) SUMO1 polymorphisms are associated with non-syndromic cleft lip with or without cleft palate. Biochem Biophys Res Commun. 377(4): 1265-8.
  • Katayama A, et al. (2007) Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: possible involvement in tumor proliferation and prognosis. Int J Oncol. 31(3): 517-24.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SUMO1 Antibody Rabbit Monoclonal ELISA 13095-R003
    Anti-SUMO1 Antibody Rabbit Polyclonal ELISA 13095-RP01
    info info
    添加购物车成功! 添加购物车失败!