SUMO1 / SUMO-1 Antibody

SUMO1 / SUMO-1 Antibody Datasheet

SUMO1 / SUMO-1 Antibody Product Information

• Immunogen: Recombinant Human SUMO1 / SUMO-1 protein (Catalog#13095-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human SUMO1 / SUMO-1 (rh SUMO1 / SUMO-1; Catalog#13095-H07E; AAH66306.1; Ser 2-Val 101). Total IgG was purified by Protein A affinity chromatography.

SUMO1 / SUMO-1 Antibody Usage Guide

• Specificity: Human SUMO1 / SUMO-1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SUMO1 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SUMO1. The detection limit for Human SUMO1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SUMO1 / SUMO-1 Antibody Background

Small ubiquitin-like modifier 1 (SUMO1) and its homologs can be conjugated to a large number of cellular proteins. This involves an enzymatic cascade that resembles ubiquitination, and the modification can be reverted by isopeptidases. SUMO1 is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). SUMO1 protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.

References

1. Pichler A. et al., 2002, Traffic. 3 (6): 381-7.
2. Zuo Y. et al.. 2009, Asian J androl. 11 (1): 36-8.
3. Kim HJ. et al., 2011, Biochem Biophys Res Commun. 410 (3): 555-62.