SULT2B1

SULT2B1 is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.

SULT2B1 Related Areas

Enzyme>>Sulfatase & Sulfotransferase>>SULT2B1

SULT2B1 Alternative Names

SULT2B1, ST2B1, HSST2 [Homo sapiens]

Sult2b1, ST2B1, AI326997, BB173635, SULT2B [Mus musculus]

Summaries for SULT2B1

Entrez Gene summary for SULT2B1:

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT2B1 gene structure (number and length of exons) is similar among family members. SULT2B1 gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

Wikipedia summary for SULT2B1:

Sulfotransferase family cytosolic 2B member 1 is an enzyme that in humans is encoded by the SULT2B1 gene.
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic chemical compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.

Human SULT2B1 Protein General Information

 

• Protein names: Sulfotransferase family cytosolic 2B member 1
  Short name=SULT2B1
• Sequence length: 365 AA.
• Catalytic activity: 3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate.
• Sequence similarities: Belongs to the sulfotransferase 1 family.
• Biophysicochemical properties: Kinetic parameters:
  K_M=10.9 µM for DHEA (at 37 degrees Celsius, in the presence of 1mM MgCl2)
  K_M=3.8 µM for DHEA (at 37 degrees Celsius, in the presence of 10mM MgCl2)
  K_M=11.8 µM for pregnenolone (at 37 degrees Celsius, in the presence of 1mM MgCl2)
  K_M=0.6 µM for PAPS (at 37 degrees Celsius, in the presence of 1mM MgCl2)
  V_max=1752 pmol/min/mg enzyme toward DHEA (at 37 degrees Celsius, in the presence of 10mM MgCl2)
  Temperature dependence:
  Optimum temperature is 37 degrees Celsius. Retains 70% and 20% of activity when incubated at 42 degrees Celsius for 45 and 120 minutes, respectively. Activity is lost after 200 minutes incubation at 42 degrees Celsius.
• Subcellular location: Cytoplasm. Microsome. Nucleus. Note: Phosphorylation of Ser-348 is required for translocation to the nucleus.
• Tissue specificity: Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.

General information above from UniProt

Function for SULT2B1 Protein

UniProtKB:

SULT2B1 is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.

Genatlas:

• SULT2B1 is hydroxysteroid sulfotransferase, regulating the activity of adrenal androgens in tissues via their inactivation by sulfation
• SULT2B1 may be playing a regulatory role that protect against the mitogenic effects of androgens

Homology for human SULT2B1

• homolog to murine Sult2b1