Anti-SULT2B1 Antibody

Cat: 11410-R007
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Anti-SULT2B1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-SULT2B1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SULT2B1
No cross-reactivity in ELISA with Human SULT2A1
Immunogen
Recombinant Human SULT2B1 Protein (Catalog#11410-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SULT2B1 (rh SULT2B1; Catalog#11410-H07E; NP_004596.2; Asp 2-Ser 365) .
Source
Monoclonal Rabbit IgG Clone #007
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SULT2B1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SULT2B1 Antibody (Rabbit Monoclonal antibody) Images
Human SULT2B1 Immunoprecipitation(IP) 6184

SULT2B1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-SULT2B1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SULT2B1 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 41 kDa

Observed band size: 41 kDa

Human SULT2B1 Western blot (WB) 13399

Anti-SULT2B1 rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:41 kDa

Observed band size:41 kDa

Anti-SULT2B1 Antibody Alternative Names
Anti-HSST2 Antibody;Anti-SULT2B1 Antibody
SULT2B1 Background Information

Sulfotransferase family cytosolic 2B member 1, also known as Sulfotransferase 2B1, ST2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, SULT2B1 and HSST2, is a cytoplasm protein which belongs to the sulfotransferase 1 family. The human hydroxysteroid sulfotransferase (SULT) family is comprised of two subfamilies, SULT2A1 and SULT2B1. SULT2B1 is expressed highly in placenta, prostate and trachea. A lesser expression of SULT1B1 was observed in the small intestine and lung. SULT2B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol. The two SULT2B1 isoforms, SULT2B1a and SULT2B1b, are encoded by a single gene as a result of alternative transcription initiation and alternative splicing. SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation.

Full Name
sulfotransferase family 2B member 1
References
  • Fujita K. et al., 1997, J. Biochem. 122:1052-61.
  • Geese,WJ. et al., 2001,Biochem Biophys Res Commun.288 (1): 280-9.
  • Shimizu,C. et al., 2003, Endocrinology. 144 (4):1186-93.
  • Ji,Y. et al., 2007, J Pharmacol Exp Ther. 322 (2): 529-40.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SULT2B1 Antibody Rabbit Polyclonal WB,ELISA 11410-RP02
    Anti-SULT2B1 Antibody Mouse Monoclonal ELISA 11410-MM04
    Anti-SULT2B1 Antibody Rabbit Polyclonal ELISA 11410-RP01
    Anti-SULT2B1 Antibody Mouse Monoclonal ELISA(Cap) 11410-MM03
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