Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
No cross-reactivity with Human SULT1B1 in ELISA
Recombinant Human SULT2A1 protein (Catalog#11411-H07E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT2A1 (rh SULT2A1; Catalog#11411-H07E; NP_003158.2; Ser 2-Glu 285). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #9G8G12G10
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) Images
Anti-SULT2A1 mouse monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:34 kDa
Observed band size:35 kDa
Anti-SULT2A1 Antibody Alternative Names
Anti-DHEA-ST Antibody;Anti-DHEAS Antibody;Anti-HST Antibody;Anti-hSTa Antibody;Anti-ST2 Antibody;Anti-ST2A1 Antibody;Anti-ST2A3 Antibody;Anti-STD Antibody
SULT2A1 Background Information
Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.
sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
Chatterjee, B. 2005, Methods Enzymol. 400:165-91. Liu,Y. et al., 2006, Chem Res Toxicol. 19 (11):1420-5. Sinclair, PA. et al., 2006, J Mol Endocrinol 36 (2):301-11. Yalcin, EB. et al., 2008, Drug Metab Lett 2 (3):198-204.