Anti-SULT2A1 Antibody

Cat: 11411-MM05
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Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-SULT2A1 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human SULT2A1
No cross-reactivity with Human SULT1B1 in ELISA
Immunogen
Recombinant Human SULT2A1 protein (Catalog#11411-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT2A1 (rh SULT2A1; Catalog#11411-H07E; NP_003158.2; Ser 2-Glu 285). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9G8G12G10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SULT2A1 Antibody (Mouse Monoclonal antibody) Images
Human SULT2A1 Western blot (WB) 13401

Anti-SULT2A1 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:35 kDa

Anti-SULT2A1 Antibody Alternative Names
Anti-DHEA-ST Antibody;Anti-DHEAS Antibody;Anti-HST Antibody;Anti-hSTa Antibody;Anti-ST2 Antibody;Anti-ST2A1 Antibody;Anti-ST2A3 Antibody;Anti-STD Antibody
SULT2A1 Background Information

Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.

Full Name
sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
References
  • Chatterjee, B. 2005, Methods Enzymol. 400:165-91.
  • Liu,Y. et al., 2006, Chem Res Toxicol. 19 (11):1420-5.
  • Sinclair, PA. et al., 2006, J Mol Endocrinol  36 (2):301-11.
  • Yalcin, EB. et al., 2008, Drug Metab Lett  2 (3):198-204.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SULT2A1 Antibody Rabbit Polyclonal WB,ELISA 11411-RP02
    Anti-SULT2A1 Antibody Rabbit Polyclonal ELISA 11411-RP01
    Anti-SULT2A1 Antibody Rabbit Monoclonal ELISA 11411-R004
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