Human SULT1E1 ORF Expression Clone, untagged

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG11275-M-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SULT1E1 cDNA Clone Datasheet

SULT1E1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005420.2
cDNA Size:	885
Gene Synonym:	EST; STE; EST-1; MGC34459; SULT1E1
Source:	Human

SULT1E1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens sulfotransferase family 1E, estrogen-preferring, member 1 DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

SULT1E1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SULT1E1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SULT1E1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTCTG AACTTGACTA TTATGAAAAG TTTGAAGAAG TCCATGGGAT TCTAATGTAT
61       AAAGATTTTG TCAAATATTG GGATAATGTG GAAGCGTTCC AGGCAAGACC AGATGATCTT
121      GTCATTGCCA CCTACCCTAA ATCTGGTACA ACCTGGGTTA GTGAAATTGT GTATATGATC
181      TATAAAGAGG GTGATGTGGA AAAGTGCAAA GAAGATGTAA TTTTTAATCG AATACCTTTC
241      CTGGAATGCA GAAAAGAAAA CCTCATGAAT GGAGTAAAAC AATTAGATGA GATGAATTCT
301      CCTAGAATTG TGAAGACTCA TTTGCCACCT GAACTTCTTC CTGCCTCATT TTGGGAAAAG
361      GATTGTAAGA TAATCTATCT TTGCCGGAAT GCAAAGGATG TGGCTGTTTC CTTTTATTAT
421      TTCTTTCTAA TGGTGGCTGG TCATCCAAAT CCTGGATCCT TTCCAGAGTT TGTGGAGAAA
481      TTCATGCAAG GACAGGTTCC TTATGGTTCC TGGTATAAAC ATGTAAAATC TTGGTGGGAA
541      AAGGGAAAGA GTCCACGTGT ACTATTTCTT TTCTACGAAG ACCTGAAAGA GGATATCAGA
601      AAAGAGGTGA TAAAATTGAT ACATTTCCTG GAAAGGAAGC CATCAGAGGA GCTTGTGGAC
661      AGGATTATAC ATCATACTTC GTTCCAAGAG ATGAAGAACA ATCCATCCAC AAATTACACA
721      ACACTGCCAG ACGAAATTAT GAACCAGAAA TTGTCGCCCT TCATGAGAAA GGGAATTACA
781      GGAGACTGGA AAAATCACTT TACAGTAGCC CTGAATGAAA AATTTGATAA ACATTATGAG
841      CAGCAAATGA AGGAATCTAC ACTGAAGTTT CGAACTGAGA TCTAA

Quality Control:cDNA sequencing results is available upon request.