SULT1B1 / ST1B2 Protein

Sulfotransferase Family Cytosolic 1B Member 1 Protein

SULT1B1 / ST1B2 Protein Product Information

• Synonym: MGC13356, ST1B1, ST1B2, SULT1B2
• Protein Construction: A DNA sequence encoding the human SULT1B1 ( NP_055280.2 ) ( Leu 2 - Ile 296 ) was expressed, with a N-terminal polyhistidine tag
• Source: Human
• Expression Host: E. coli

SULT1B1 / ST1B2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human SULT1B1 comprises 302 amino acids and has a calculated molecular mass of 35.7 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris, 0.1 M nacl, 10% glycerol, 1mM DTT, pH 8.0
• SDS-PAGE: SULT1B1 protein

SULT1B1 / ST1B2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SULT1B1 / ST1B2 Protein Description

Sulfotransferase family cytosolic 1B member 1, also known as Sulfotransferase 1B1, Sulfotransferase 1B2, Thyroid hormone sulfotransferase, SULT1B1 and ST1B2, is a cytoplasm protein which belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver, peripheral blood leukocytes, colon (mucosal lining), small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung, placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine, reverse triiodothyronine and thyroxine.

References

1. Fujita K. et al., 1997, J. Biochem. 122:1052-61.
2. Kester,MH.et al., 2003,Am J Physiol Endocrinol Metab. 285 (3):E592-8.
3. Meinl W, et al., 2001, Biochem Biophys Res Commun. 288 (4): 855-62.
4. Dombrovski L. et al., 2006, Proteins 64: 1091-4.