SULT1B1 Antibody (Antigen Affinity Purified)

SULT1B1 Antibody ( Antigen Affinity Purified ) Datasheet

SULT1B1 Antibody Product Information

• Immunogen: Recombinant Human SULT1B1 protein (Catalog#11592-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human SULT1B1 (rh SULT1B1; Catalog#11592-H07E; NP_055280.2; Leu 2-Ile 296). SULT1B1 specific IgG was purified by Human SULT1B1 affinity chromatography.

SULT1B1 Antibody Usage Guide

• Specificity: Human SULT1B1
• Immunochemistry: IHC-P: 0.1-1 μg/mL

  

  Immunochemical staining of human SULT1B1 in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  

  Immunochemical staining of human SULT1B1 in human spleen with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SULT1B1 in WB. Using a DAB detection system, the detection limit for Human SULT1B1 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SULT1B1. The detection limit for Human SULT1B1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SULT1B1 Antibody Background

Sulfotransferase family cytosolic 1B member 1, also known as Sulfotransferase 1B1, Sulfotransferase 1B2, Thyroid hormone sulfotransferase, SULT1B1 and ST1B2, is a cytoplasm protein which belongs to thesulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver, peripheral blood leukocytes, colon (mucosal lining), small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung, placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine, reverse triiodothyronine and thyroxine.

References

1. Fujita K. et al., 1997, J. Biochem. 122:1052-61.
2. Kester,MH.et al., 2003,Am J Physiol Endocrinol Metab. 285 (3):E592-8.
3. Meinl W, et al., 2001, Biochem Biophys Res Commun. 288 (4): 855-62.
4. Dombrovski L. et al., 2006, Proteins 64: 1091-4.