SULT1A1 Antibody (Antigen Affinity Purified)

SULT1A1 Antibody ( Antigen Affinity Purified ) Datasheet

SULT1A1 Antibody Product Information

• Immunogen: Recombinant Human SULT1A1 protein (Catalog#11658-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose

SULT1A1 Antibody Usage Guide

• Specificity: Human SULT1A1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human SULT1A1 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human SULT1A1 in human prostatic carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SULT1A1 in WB. Using a DAB detection system, the detection limit for Human SULT1A1 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SULT1A1. The detection limit for Human SULT1A1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SULT1A1 Antibody Background

Sulfotransferases (SULTs) are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 (SULT1A1) belongs to the sulfotransferase 1 subfamily. Members of the SULT1 family have demonstrated the ability to sulfonate simple (small planar) phenols including estradiol, thyroid hormones, environmental xenobiotics and drugs. SULT1A1 mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences, including the influence of SULT1A1 genotype on susceptibility to colorectal and breast cancer. Furthermore, an Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer).

References

1. Klaassen CD. et al., 1998, Chem Biol Interact. 109 (1-3): 299-313.
2. Glatt H. 2000, Chem Biol Interact. 129 (1-2): 141-70.
3. Lindsay J. et al., 2008, Curr Drug Metab. 9 (2): 99-105.
4. Coughtrie MW. 2002, Pharmacogenomics J. 2 (5): 297-308.
5. Glatt H. et al., 2004, Naunyn Schmiedebergs Arch Pharmacol. 369 (1): 55-68.