Mouse STYK1/NOK Gene cDNA Clone / ORF Clone

STYK1 cDNA Clone Datasheet

STYK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_172891.2
• cDNA Size: 1290
• Gene Synonym: Nok; AI326477; 9130025L13; Styk1
• Source: Mouse

STYK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serine/threonine/tyrosine kinase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for five point mutations: 71 G/A resulting in the amino acid Arg substitution by Gln， 418 A/G resulting in the amino acid Ser substitution by Gly， 510 C/A, 643 C/A, 1032 A/G not causing the amino acid variation.

STYK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

STYK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

STYK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGAGA AGGGTCACCT GAGCCGGGTG TTGTTGGAAT GCAGTCTCAG TGACAAGCTG
61       TGTGTTGTCC AAGAGAAGCA GTATGAAGTA ATCATTGTTC CGGCTCTCCT GGTTGGCGGC
121      TTTCTCATCC TTCTTGCAAT CATCCTGTGG CTTTTTATCA GAGGACAGAG ATCCCAAAGG
181      CAGAGCCCTG GACCCCGAGG CACTGCCTCT GTACCTGCAT CTAGGGGCAG AAGCCAGGAG
241      GCAGCAGGAC ATGGAGAAAA AGTACTTCTG CCTCTTAAGG AGACCTCAGT GGAGGGCTTT
301      CTGCGAGCGG CCACACCCCG CCTGGCTAAG CTGCAGGTGC CCAGGGAACA GCTCTTGGAG
361      GTTCTGGAGC AAATCCACAG TGGTAGTTGT GGGACCCTCT ATCATGCAAC AATGACCGCC
421      AAGGATCACC CTAAGCCAAA AAGTGTGGTC CTTAAGGCTT TAGAAGATCC AGTTGGACTC
481      CAGGAGGTCC AGGATTTTAT AGGACGAATA CAATTCTATC AGTACCTGGG GAAACACAAG
541      AACCTGGTAC AGCTGGAGGG CTGCTGTACA GAAAGGCTGC CACTCTATAT GATGCTGGAG
601      GATGTGGTCC CAGGGGACCT GCTCAGCTTT CTTTGGACCT GTAGAAGGGA TGTGATGACC
661      ATGGATGGCC TGCTCTATGA TCTCACAGAA AAACAAATAT ATCACATTGG AAAGCAGATC
721      CTTTTGGCCC TGGAATTCCT GCAGGAGAAG CACCTGTTTC ATGGGGATGT GGCTGCCAGG
781      AACATCCTGA TCCAAAGTGA CCTGACTCCC AAACTTTGTC ATCTGGGCCT GGCTTATGAA
841      GTTCATGCCC ATGGGGCCAT CTCCTCTGCT CGATCCAGCA CCATCCCTCT CAAGTGGCTT
901      GCTCCAGAAA GGCTTCTCCT GAGACCTGCA AGCATCAGGG GAGATATTTG GTCCTTTGGG
961      ATCCTGCTTT ATGAGATGGT GACTCTAGGA GCACCACCAT ACCCTGAAGT CCCTCCCACC
1021     AGCATCCTAC AGTATCTTCA GAGAAAGAAA ATCATGAAGA GACCCAGCAG CTGCTCACAT
1081     GCCATGTACA ACATCATGAA GTGCTGTTGG CGCTGGAGTG AGGACAGCCG CCCCTTACTT
1141     GGTCAGCTGC TCCAGCGCCT AGAAGCTGCT TCTAGATCTG CCGATGACAA GGCTGTGTTG
1201     CAAGTGCCAG AGTTGGTGGT GCCTGAACTG TATGCAGATG TGGCTGGCAT CAGGGCAGAA
1261     AGCATTTCCT ATAGCTTCAG TGTCCTTTGA

 

Quality Control:cDNA sequencing results is available upon request.