STK40 Protein (GST Tag)

Serine / threonine kinase 40 Protein Datasheet

STK40 Protein Product Information

• Synonym: SGK495, SHIK, STK40
• Protein Construction: A DNA sequence encoding the human STK40 (NP_114406) (Met1-Lys435) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

STK40 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human STK40 /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl,10%glycerol,3mM DTT, 0.5M Urea, 0.5mM GSH, pH 8.0.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: STK40 protein

STK40 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

STK40 Protein Description

STK40 may be a negative regulator of NF-kappa-B and p53-mediated gene transcription. It localized to both the cytoplasm and the nucleus. It is strongly expressed in heart, brain, placenta, lung, skeletal muscle, kidney, spleen, thymus, prostate, liver, pancreas, testis, ovary, small intestine, colon and peripheral blood leukocytes. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. It interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription.

References

1. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
2. Wiemann S. et al., 2001, Genome Res. 11 (3): 422-35.
3. Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.