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STK4 / MST1 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11533 |
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Human STK4 / MST1 ELISA Pair Set
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Order or Inquire for STK4 / MST1 ELISA Pair Set |
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High affinity Human STK4 / MST1 ELISA Pair Set |
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Detection limit - 78.125 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human STK4 / MST1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Serine/threonine-protein kinase 4, also known as Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2 STK4 and MST1, is a cytoplasm and nucleus protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. STK4 / MST-1 is ubiquitously expressed. It contains oneprotein kinase domain and oneSARAH domain. STK4 / MST-1 is stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. STK4 / MST-1 is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. STK4 / MST-1 phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. STK4 / MST-1 phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
