STK4 / MST1 Antibody

STK4 / MST1 Antibody Datasheet

STK4 / MST1 Antibody Product Information

• Immunogen: Recombinant Human STK4 / MST1 protein (Catalog#11533-H07B)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 1G5H4
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human STK4 / MST1 (rh STK4 / MST1; Catalog#11533-H07B; Q13043-1; Glu 2-Phe 487). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

STK4 / MST1 Antibody Usage Guide

• Specificity: Human STK4 / MST1
• No cross-reactivity in ELISA with

  Baculovirus lysate

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human STK4 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human STK4. The detection limit for Human STK4 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

STK4 / MST1 Antibody Background

Serine/threonine-protein kinase 4, also known as Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2 STK4 and MST1, is a cytoplasm and nucleus protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. STK4 / MST-1 is ubiquitously expressed. It contains oneprotein kinase domain and oneSARAH domain. STK4 / MST-1 is stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. STK4 / MST-1 is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. STK4 / MST-1 phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. STK4 / MST-1 phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.

References

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2. Taylor L.K.et al., 1996, Proc. Natl. Acad. Sci. USA. 93: 10099-104.
3. Creasy C.L.et al., 1996, J. Biol. Chem. 271: 21049-53.
4. Oh H.J. et al., 2006, Cancer Res. 66: 2562-9.
5. Lehtinen M.K.et al., 2006, Cell 125: 987-1001.