Mouse STK25 Gene cDNA Clone / ORF Clone

STK25 cDNA Clone Datasheet

STK25 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021537.3
• cDNA Size: 1281
• Gene Synonym: Ysk1; C86992; AU018434; Ste20-like; 1500019J11Rik; Stk25
• Source: Mouse

STK25 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serine/threonine kinase 25 (yeast) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 960 G/A not causing the amino acid variation.

STK25 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

STK25 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

STK25 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCACC TCCGGGGCTT CGCGCACCAG CATTCTCGTG TGGACCCAGA GGAACTCTTC
61       ACCAAGCTTG ACCGCATTGG CAAAGGCTCA TTTGGGGAGG TGTACAAGGG GATCGACAAC
121      CACACCAAGG AAGTGGTGGC CATCAAGATC ATTGACTTGG AAGAGGCCGA GGATGAAATT
181      GAGGACATCC AACAGGAGAT CACTGTGCTC AGCCAATGTG ACAGTCCTTA TATCACCCGC
241      TACTTCGGCT CCTACCTGAA GAGCACCAAG CTGTGGATTA TCATGGAGTA CCTGGGTGGT
301      GGCTCTGCAC TGGACTTGCT GAAACCTGGT CCCCTGGAAG AGACCTATAT TGCCACCATC
361      TTGCGGGAGA TCCTGAAAGG CCTGGATTAT CTGCACTCAG AGCGCAAGAT CCACCGAGAT
421      ATCAAAGCTG CCAACGTGCT GCTCTCGGAG CAGGGTGATG TGAAGCTGGC AGACTTCGGT
481      GTGGCTGGGC AGCTCACAGA CACACAAATC AAGAGGAACA CATTTGTGGG TACACCCTTC
541      TGGATGGCAC CTGAGGTTAT CAAGCAGTCG GCCTATGACT TCAAGGCTGA CATCTGGTCC
601      CTGGGCATCA CAGCCATTGA GCTGGCCAAG GGGGAGCCGC CAAACTCTGA TCTCCACCCT
661      ATGCGAGTCC TGTTCCTGAT TCCCAAGAAC AACCCACCCA CATTGGAGGG CCACCACAGC
721      AAACCCTTCA AGGAGTTTGT AGAGGCCTGC CTCAATAAGG ACCCACGATT CCGACCCACT
781      GCCAAGGAGC TCCTGAAGCA CAAATTCATC ACACGATACA CCAAGAAGAC CTCCTTTCTC
841      ACTGAACTTA TTGACCGATA TAAGCGCTGG AAGTCTGAGG GGCATGGAGA GGAGTCCAGT
901      TCTGAGGATT CTGACATTGA TGGCGAGGCT GAGGATGGGG AGCAGGGTCC CATCTGGACA
961      TTTCCTCCAA CCATCCGGCC AAGTCCACAT AGCAAGCTGC ACAAGGGAAC TGCTCTGCAC
1021     AGCTCACAGA AGCCTGCAGA GCCTATCAAG AGACAGCCAA GGTCCCAGTG CTTGTCCACA
1081     CTAGTCCGTC CTGTCTTTGG AGAGCTCAAA GAGAAGCACA AGCAGAGCGG TGGGAGCGTG
1141     GGTGCCCTGG AGGAGCTAGA GAATGCCTTC AGCCTGGCTG AGGAATCCTG TCCTGGCATC
1201     TCAGACAAGC TGATGGTGCA CCTGGTGGAG CGTGTGCAGA GGTTCTCACA CAGCAGGAAC
1261     CACCTGACAT CTACCCGCTG A

 

Quality Control:cDNA sequencing results is available upon request.