STK24 / MST3 Protein Price Inquiry ( Available Sizes )
STK24 / MST3 Protein Product Information
||RP11-111L24.5, MST3, MST3B, STE20, STK3
||A DNA sequence encoding the human STK24 isoform A (Q9Y6E0-2) (Met 1- His 431) was expressed, with a polyhistidine tag at the N-terminus.
STK24 / MST3 Protein QC Testing
||> 82 % as determined by SDS-PAGE.
STK24 / MST3 protein
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||The recombinant human STK24 consists of 450 amino acids and predicts a molecular mass of 50.3 kDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.
||Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20%gly, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTT
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
STK24 / MST3 Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
STK24 / MST3 Protein Related Products & Topics
STK24 / MST3 Protein Description
Serine / threonine-protein kinase 24 (STK24) or mammalian ste20-like kinase 3 (MST3), is a member of the Serine / threonine-protein kinase family with a unique preference for manganese ion as a cofactor. The yeast sterile-20 functions upstream of the mitogen-activated protein kinase (MAPK) cascade. Four metal ions (Mg2+, Mn2+, Zn2+, and Co2+) activate endogenous, exogenous, and baculovirus-expressed recombinant MST3 within the physiological concentration range. In contrast, Fe2+ and Ca2+ do not function as MST3 cofactors. MST3 autophosphorylation is on both serine and threonine residues. The distinct autophosphorylation pattern on MST3 suggests that MST3 may exert various types of kinase reactions depending on the type of metal ion cofactor used. MST3 may inhibit cell migration in a fashion dependent on autophosphorylation and may regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST.
- Lu TJ. et al., 2006, J Biol Chem. 281 (50): 38405-17.
- Lu TJ. et al., 2005, J Inorg Biochem. 99 (6): 1306-13.
- Schinkmann K. et al., 1997, J Biol Chem. 272 (45): 28695-703.