STK10 (LOK)

STK10 is serine/threonine-protein kinase involved in regulation of lymphocyte migration. STK10 phosphorylates MSN, and possibly PLK1. STK10 is involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. STK10 acts as a negative regulator of MAP3K1/MEKK1. STK10 may also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.

STK10 (LOK) Related Areas

Enzyme>>Protein Kinase>>Intracellular Kinase>>STK10/LOK

Signal Transduction>>Protein Kinase>>Intracellular Kinase>>STK10/LOK

STK10 (LOK) Alternative Names

STK10, LOK, PRO2729 [Homo sapiens]

Stk10, Lok,, RP23-395O6.3, Gek1, KIAA4026, mKIAA4026 [Mus musculus]

Summaries for STK10 (LOK)

Entrez Gene summary for STK10 (LOK):

STK10 gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]

Wikipedia summary for STK10 (LOK):

Serine/threonine-protein kinase 10 is an enzyme that in humans is encoded by the STK10 gene.
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway.

Human STK10 (LOK) Protein General Information

 

• Protein names: Serine/threonine-protein kinase 10
  Short name=STK10
• Sequence length: 968 AA.
• Domain: Coiled coil
• Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
  Contains 1 protein kinase domain.
• Post-translational modification: Autophosphorylates following homodimerization, leading to activation of the protein.
• Subunit structure: Homodimer; homodimerization is required for activation segment autophosphorylation.
• Subcellular location: Cell membrane; Peripheral membrane protein
• Tissue specificity: Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.
• Involvement in disease: Defects in STK10 may be a cause of testicular germ cell tumor (TGCT) . A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
• Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
• Enzyme regulation: Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib.
• Miscellaneous: Inhibition by erlotinib, an orally administered EGFR tyrosine kinase inhibitor used for treatment, enhances STK10-dependent lymphocytic responses, possibly leading to the aggravation of skin inflammation observed upon treatment by erlotinib
• Sequence caution: The sequence BAG51143.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

General information above from UniProt

Function for STK10 (LOK) Protein

UniProtKB:

STK10 is serine/threonine-protein kinase involved in regulation of lymphocyte migration. STK10 phosphorylates MSN, and possibly PLK1. STK10 is involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. STK10 acts as a negative regulator of MAP3K1/MEKK1. STK10 may also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.

Genatlas:

• STK10 acts on substrates such as myelin basic protein and histone 2A on serine and threonine residues
• STK10 is polo-like kinase kinase that cooperates with SLK to regulate PLK1 function in human cells
• STK10 plays a role in ERM ((ezrin-radixin-moesin) phosphorylation, and major ERM kinase in lymphocytes

Homology for human STK10 (LOK)

• homolog to murine Stk10