Mouse STIM1 Gene cDNA Clone / ORF Clone

STIM1 cDNA Clone Datasheet

STIM1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009287.4
• cDNA Size: 2058
• Gene Synonym: SIM; Stim1
• Source: Mouse

STIM1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus stromal interaction molecule 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for four point mutations: 375 G/A, 456 A/G, 837 G/A, 878 G/A not causing the amino acid variation.

STIM1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

STIM1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

STIM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATGTGT GCGCCCGTCT TGCCCTGTGG CTTCTTTGGG GGCTCCTTCT GCATCAGGGC
61       CAGAGTCTCA GCCATAGTCA CAGTGAAAAG AATACAGGAG CTAGCTCCGG GGCGACTTCT
121      GAAGAGTCTA CCGAAGCAGA GTTTTGCCGA ATTGACAAGC CCCTGTGCCA CAGTGAGGAT
181      GAGAAGCTCA GCTTTGAGGC CGTCCGAAAC ATCCATAAGC TGATGGATGA CGATGCCAAT
241      GGTGATGTGG ATGTGGAAGA AAGTGATGAG TTCCTAAGGG AAGACCTCAA TTACCATGAC
301      CCAACAGTGA AACATAGCAC CTTCCATGGT GAGGATAAGC TTATCAGCGT GGAGGACCTG
361      TGGAAGGCGT GGAAATCATC AGAAGTGTAC AACTGGACTG TGGATGAGGT GATACAGTGG
421      CTCATTACGT ATGTGGAGCT GCCACAGTAT GAGGAGACCT TCCGGAAGTT GCAGCTTACT
481      GGCCACGCCA TGCCAAGGCT AGCAGTAACC AACACCACCA TGACAGGGAC TGTACTGAAG
541      ATGACAGATC GGAGCCACAG GCAGAAGCTG CAGCTGAAGG CCCTGGACAC AGTGCTGTTT
601      GGGCCTCCTC TCTTGACTCG GCATAATCAC CTGAAGGACT TCATGCTGGT GGTGTCTATC
661      GTTATTGGTG TGGGTGGCTG CTGGTTTGCC TATATCCAGA ACCGTTACTC TAAGGAGCAC
721      ATGAAGAAAA TGATGAAGGA TCTGGAAGGG TTACACCGGG CTGAGCAGAG TCTGCATGAC
781      CTTCAGGAAA GGCTGCACAA GGCCCAGGAG GAGCACCGAA CTGTGGAAGT AGAGAAAGTC
841      CACCTGGAGA AGAAGCTGCG AGATGAGATC AACCTTGCCA AGCAGGAAGC TCAGCGGCTG
901      AAGGAGCTGA GGGAGGGTAC TGAGAATGAG AGGAGCCGTC AAAAATATGC TGAGGAAGAG
961      CTGGAGCAGG TTCGGGAGGC CTTGAGGAAA GCAGAGAAGG AGCTGGAATC ACACAGCTCA
1021     TGGTATGCTC CTGAGGCCCT GCAGAAGTGG CTGCAGCTGA CCCATGAGGT GGAGGTGCAG
1081     TACTACAACA TCAAGAAGCA AAATGCAGAG AGGCAGCTGC TGGTGGCCAA GGAGGGGGCT
1141     GAGAAAATAA AAAAGAAGAG AAACACGCTT TTTGGTACCT TCCATGTGGC CCACAGCTCT
1201     TCCCTGGATG ATGTGGATCA TAAAATCCTA ACTGCTAAGC AAGCTCTGAG TGAGGTGACA
1261     GCGGCACTGA GGGAGCGCCT GCACCGGTGG CAGCAGATCG AGATCCTCTG CGGTTTCCAG
1321     ATTGTCAATA ACCCCGGCAT CCACTCCTTG GTGGCTGCTC TCAACATCGA CCCCAGCTGG
1381     ATGGGCAGCA CCCGCCCTAA CCCCGCCCAC TTCATCATGA CTGACGATGT GGATGACATG
1441     GATGAGGAGA TTGTGTCGCC CTTGTCCATG CAGTCCCCCA GCCTGCAGAG CAGTGTCCGG
1501     CAGCGCCTGA CGGAGCCACA GCTTGGCCTG GGATCTCAGA GGGATTTGAC CCATTCCGAT
1561     TCGGAGTCCT CCCTCCACAT GAGTGACCGC CAGCGTGTGG CCCCCAAGCC TCCTCAGATG
1621     GGCCGTGCTG CAGATGAAGC TCTCAATGCC ATGCCTTCCA ATGGCAGCCA TCGGCTGATT
1681     GAGGGGGTCC ATCCAGGATC TCTGGTGGAG AAACTGCCTG ACAGCCCTGC TCTGGCCAAG
1741     AAGACATTTA TGGCGTTGAA CCATGGCCTA GACAAGGCCC ACAGCCTGAT GGAGCTGAAC
1801     CCCTCAGTCC CACCTGGTGG CTCCCCACTT TTGGATTCTT CCCATTCTCT TAGCCCCAGT
1861     TCCCCAGACC CAGACACACC ATCTCCAGTT GGGGACAACC GAGCTCTGCA GGGTAGCCGA
1921     AACACACGAA TTCCCCACTT GGCTGGCAAG AAGGCAATGG CTGAGGAGGA TAATGGTTCC
1981     ATTGGTGAGG AGACAGACTC CAGTCCAGGC AGGAAGAAGT TTCCTCTCAA AATTTTTAAG
2041     AAGCCTCTTA AGAAGTAG

 

Quality Control:cDNA sequencing results is available upon request.