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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human STATH Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13694-G-F|
|Human STATH Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13694-G-H|
|Human STATH Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13694-G-M|
|Human STATH Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13694-G-N|
|Human STATH Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13694-G-Y|
Statherin, also known as STATH, belongs to the histatin/statherin family. Statherin may play an important role in the maintenance of oral health.It prevents calcium phospate precipitation in saliva, so maintaining a high calcium level in saliva and preventing teeth from dissolving. Statherin also inhibits spontaneous precipitation of calcium phosphate salts. Thus, statherin and PRPs may prevent build-up of harmful deposits in the salivary glands and on the tooth surfaces.Statherin is a highly stable salivary protein of low molecular mass (5,380). Sabatini et al. synthesized mixed oligonucleotides based on the known amino acid sequence of statherin and used these to screen a cDNA library constructed from human parotid gland mRNA.