STAT4 Protein (His Tag)

Signal transducer and activator of transcription 4 Protein Datasheet

STAT4 Protein Product Information

• Synonym: SLEB11
• Protein Construction: A DNA sequence encoding the human STAT4 (Q14765) (Met 1- Glu 748) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

STAT4 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human STAT4 consists of 767 amino acids and predicts a molecular mass of 88 kDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20%gly, 0.5mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: STAT4 protein

STAT4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

STAT4 Protein Description

The STAT protein 4 (Signal Transducer and Activator of Transcription, or Signal Transduction and transcription, or STAT4) is one STAT family member that specifically mediate signals that emanate from the IL-12 receptors and play a specific role in lymphocyte function. STAT4 was initially cloned as a result of its homology with STAT1 and is widely expressed, although it is only tyrosine- or serine- phosphorylated after stimulation of T cells with interleukin (IL) -12 and IFN-α. STAT4 plays an important role in Th1 immune responses. STAT4 is activated after IL-12 interacts with the IL-12 receptor, including transcription of IFN-λ. Studies has documented the association of a common STAT4 haplotype (tagged by rs7574865) with risk for rheumatoid arthritis (RA) and systemic lupus erythematosus in both Asian and White populations.

References

1. Thierfelder WE. et al., 1996, Nature. 382 (6587): 171-4.
2. Hye-Soon L. et al., 2007, Mol Med. 13 (9-10): 455-60.
3. Chitnis T. et al., 2001, J Clin Invest. 108 (5): 739-47.