Anti-ST6GAL1 Antibody

Cat: 11590-RP02
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Anti-ST6GAL1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ST6GAL1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human ST6GAL1
Immunogen
Recombinant Human ST6GAL1 / ST6GAL-I protein (Catalog#11590-H07H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ST6GAL1 / ST6GAL-I (rh ST6GAL1 / ST6GAL-I; Catalog#11590-H07H; P15907; Lys 27-Cys406). ST6GAL1 / ST6GAL-I specific IgG was purified by Human ST6GAL1 / ST6GAL-I affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ST6GAL1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ST6GAL1 Antibody (Rabbit Polyclonal antibody) Images
Human ST6GAL1/ST6GAL-I Immunoprecipitation(IP) 8095

ST6GAL1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-ST6GAL1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ST6GAL1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 47 kDa

Observed band size: 47 kDa

Human ST6GAL1/ST6GAL-I Western blot (WB) 13419

Anti-ST6GAL1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:47 kDa

Observed band size:55 kDa

Anti-ST6GAL1 Antibody Alternative Names
Anti-SIAT1 Antibody;Anti-ST6GalI Antibody;Anti-ST6N Antibody
ST6GAL1 Background Information

Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

Full Name
ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
References
  • Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206.
  • Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68.
  • Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414.
  • Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ST6GAL1 Antibody Rabbit Polyclonal ELISA 11590-RP01
    Anti-ST6GAL1 Antibody Mouse Monoclonal ELISA 11590-MM05
    Anti-ST6GAL1 Antibody Rabbit Monoclonal ELISA(Cap) 11590-R001
    Anti-ST6GAL1 Antibody Rabbit Monoclonal IHC-P 11590-R036
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