Human ST3GAL3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13753-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ST3GAL3 cDNA Clone Datasheet

ST3GAL3 cDNA Clone Product Information

Gene Bank Ref.ID:	BC050380
cDNA Size:	1128
Gene Synonym:	MRT12; SIAT6; ST3GALII; ST3GalIII; ST3N; ST3GAL3
Source:	Human

ST3GAL3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1126-1128 TGA/TAA not causing the amino acid variation.

ST3GAL3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ST3GAL3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ST3GAL3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGACTCT TGGTATTTGT GCGCAATCTG CTGCTAGCCC TCTGCCTCTT TCTGGTACTG
61       GGATTTTTGT ATTATTCTGC GTGGAAGCTA CACTTACTCC AGTGGGAGGA GGACTCCAAT
121      TCAGTGGTTC TTTCCTTTGA CTCCGCTGGA CAAACACTAG GCTCAGAGTA TGATCGGTTG
181      GGCTTCCTCC TGAATCTGGA CTCTAAACTG CCTGCTGAAT TAGCCACCAA GTACGCAAAC
241      TTTTCAGAGG GAGCTTGCAA GCCTGGCTAT GCTTCAGCCT TGATGACGGC CATCTTCCCC
301      CGGTTCTCCA AGCCAGCACC CATGTTCCTG GATGACTCCT TTCGCAAGTG GGCTAGAATC
361      CGGGAGTTCG TGCCGCCTTT TGGGATCAAA GGTCAAGACA ATCTGATCAA AGCCATCTTG
421      TCAGTCACCA AAGAGTACCG CCTGACCCCT GCCTTGGACA GCCTCCGCTG CCGCCGCTGC
481      ATCATCGTGG GCAATGGAGG CGTTCTTGCC AACAAGTCTC TGGGGTCACG AATTGACGAC
541      TATGACATTG TGGTGAGACT GAATTCAGCA CCAGTGAAAG GCTTTGAGAA GGACGTGGGC
601      AGCAAAACGA CACTGCGCAT CACCTACCCC GAGGGCGCCA TGCAGCGGCC TGAGCAGTAC
661      GAGCGCGATT CTCTCTTTGT CCTCGCCGGC TTCAAGTGGC AGGACTTTAA GTGGTTGAAA
721      TACATCGTCT ACAAGGAGAG AGTGAGTGCA TCGGATGGCT TCTGGAAATC TGTGGCCACT
781      CGAGTGCCCA AGGAGCCCCC TGAGATTCGA ATCCTCAACC CATATTTCAT CCAGGAGGCC
841      GCCTTCACCC TCATTGGCCT GCCCTTCAAC AATGGCCTCA TGGGCCGGGG GAACATCCCT
901      ACCCTTGGCA GTGTGGCAGT GACCATGGCA CTACACGGCT GTGACGAGGT GGCAGTCGCA
961      GGATTTGGCT ATGACATGAG CACACCCAAC GCACCCCTGC ACTACTATGA GACCGTTCGC
1021     ATGGCAGCCA TCAAAGAGTC CTGGACGCAC AATATCCAGC GAGAGAAAGA GTTTCTGCGG
1081     AAGCTGGTGA AAGCTCGCGT CATCACTGAT CTAAGCAGTG GCATCTAA

Quality Control:cDNA sequencing results is available upon request.