SRC / Proto-oncogene c-Src Protein (GST Tag)

V-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Protein Datasheet

SRC Protein Product Information

• Synonym: RP5-823N20.1, ASV, SRC1, c-SRC, p60-Src
• Protein Construction: A DNA sequence encoding the human SRC (P12931-1) (Met 1-Leu 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells-Baculovirus-Insect cells

SRC Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7KDa. It migrates as an approximately 81 KDa band in SDS-PAGE under non-reduced conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH 8.0, 20%gly, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SRC protein

SRC Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SRC Protein Description

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

References

1. Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
2. Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
3. Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.