Human SPOP Gene cDNA Clone / ORF Clone

Human SPOP cDNA Clone Datasheet

SPOP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_003563.3
• cDNA Size: 1125
• Gene Synonym: TEF2; SPOP
• Source: Human

SPOP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens speckle-type POZ protein DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SPOP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SPOP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SPOP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCAAGGG TTCCAAGTCC TCCACCTCCG GCAGAAATGT CGAGTGGCCC CGTAGCTGAG
61       AGTTGGTGCT ACACACAGAT CAAGGTAGTG AAATTCTCCT ACATGTGGAC CATCAATAAC
121      TTTAGCTTTT GCCGGGAGGA AATGGGTGAA GTCATTAAAA GTTCTACATT TTCATCAGGA
181      GCAAATGATA AACTGAAATG GTGTTTGCGA GTAAACCCCA AAGGGTTAGA TGAAGAAAGC
241      AAAGATTACC TGTCACTTTA CCTGTTACTG GTCAGCTGTC CAAAGAGTGA AGTTCGGGCA
301      AAATTCAAAT TCTCCATCCT GAATGCCAAG GGAGAAGAAA CCAAAGCTAT GGAGAGTCAA
361      CGGGCATATA GGTTTGTGCA AGGCAAAGAC TGGGGATTCA AGAAATTCAT CCGTAGAGAT
421      TTTCTTTTGG ATGAGGCCAA CGGGCTTCTC CCTGATGACA AGCTTACCCT CTTCTGCGAG
481      GTGAGTGTTG TGCAAGATTC TGTCAACATT TCTGGCCAGA ATACCATGAA CATGGTAAAG
541      GTTCCTGAGT GCCGGCTGGC AGATGAGTTA GGAGGACTGT GGGAGAATTC CCGGTTCACA
601      GACTGCTGCT TGTGTGTTGC CGGCCAGGAA TTCCAGGCTC ACAAGGCTAT CTTAGCAGCT
661      CGTTCTCCGG TTTTTAGTGC CATGTTTGAA CATGAAATGG AGGAGAGCAA AAAGAATCGA
721      GTTGAAATCA ATGATGTGGA GCCTGAAGTT TTTAAGGAAA TGATGTGCTT CATTTACACG
781      GGGAAGGCTC CAAACCTCGA CAAAATGGCT GATGATTTGC TGGCAGCTGC TGACAAGTAT
841      GCCCTGGAGC GCTTAAAGGT CATGTGTGAG GATGCCCTCT GCAGTAACCT GTCCGTGGAG
901      AACGCTGCAG AAATTCTCAT CCTGGCCGAC CTCCACAGTG CAGATCAGTT GAAAACTCAG
961      GCAGTGGATT TCATCAACTA TCATGCTTCG GATGTCTTGG AGACCTCTGG GTGGAAGTCA
1021     ATGGTGGTGT CACATCCCCA CTTGGTGGCT GAGGCATACC GCTCTCTGGC TTCAGCACAG
1081     TGCCCTTTTC TGGGACCCCC ACGCAAACGC CTGAAGCAAT CCTAA

 

Quality Control:cDNA sequencing results is available upon request.