Human SPN Gene cDNA Clone / ORF Clone

Human SPN cDNA Clone Datasheet

SPN cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001030288.1
• cDNA Size: 1203
• Gene Synonym: LSN; CD43; GPL115; SPN
• Source: Human

SPN cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens sialophorin DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SPN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SPN cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SPN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCACGC TTCTCCTTCT CCTTGGGGTG CTGGTGGTAA GCCCAGACGC TCTGGGGAGC
61       ACAACAGCAG TGCAGACACC CACCTCCGGA GAGCCTTTGG TCTCTACTAG CGAGCCCCTG
121      AGCTCAAAGA TGTACACCAC TTCAATAACA AGTGACCCTA AGGCCGACAG CACTGGGGAC
181      CAGACCTCAG CCCTACCTCC CTCAACTTCC ATCAATGAGG GATCCCCTCT TTGGACTTCC
241      ATTGGTGCCA GCACTGGTTC CCCTTTACCT GAGCCAACAA CCTACCAGGA AGTTTCCATC
301      AAGATGTCAT CAGTGCCCCA GGAAACCCCT CATGCAACCA GTCATCCTGC TGTTCCCATA
361      ACAGCAAACT CTCTAGGATC CCACACCGTG ACAGGTGGAA CCATAACAAC GAACTCTCCA
421      GAAACCTCCA GTAGGACCAG TGGAGCCCCT GTTACCACGG CAGCTAGCTC TCTGGAGACC
481      TCCAGAGGCA CCTCTGGACC CCCTCTTACC ATGGCAACTG TCTCTCTGGA GACTTCCAAA
541      GGCACCTCTG GACCCCCTGT TACCATGGCA ACTGACTCTC TGGAGACCTC CACTGGGACC
601      ACTGGACCCC CTGTTACCAT GACAACTGGC TCTCTGGAGC CCTCCAGCGG GGCCAGTGGA
661      CCCCAGGTCT CTAGCGTAAA ACTATCTACA ATGATGTCTC CAACGACCTC CACCAACGCA
721      AGCACTGTGC CCTTCCGGAA CCCAGATGAG AACTCACGAG GCATGCTGCC AGTGGCTGTG
781      CTTGTGGCCC TGCTGGCGGT CATAGTCCTC GTGGCTCTGC TCCTGCTGTG GCGCCGGCGG
841      CAGAAGCGGC GGACTGGGGC CCTCGTGCTG AGCAGAGGCG GCAAGCGTAA CGGGGTGGTG
901      GACGCCTGGG CTGGGCCAGC CCAGGTCCCT GAGGAGGGGG CCGTGACAGT GACCGTGGGA
961      GGGTCCGGGG GCGACAAGGG CTCTGGGTTC CCCGATGGGG AGGGGTCTAG CCGTCGGCCC
1021     ACGCTCACCA CTTTCTTTGG CAGACGGAAG TCTCGCCAGG GCTCCCTGGC GATGGAGGAG
1081     CTGAAGTCTG GGTCAGGCCC CAGCCTCAAA GGGGAGGAGG AGCCACTGGT GGCCAGTGAG
1141     GATGGGGCTG TGGACGCCCC AGCTCCTGAT GAGCCCGAAG GGGGAGACGG GGCTGCCCCT
1201     TAA

 

Quality Control:cDNA sequencing results is available upon request.